卷丹侧生器官边界域基因LlLBD18的克隆和功能分析  

Cloning and Functional Analysis of LlLBD18 in Lilium lancifolium

作　　者：殷小雨 何国仁 毕蒙蒙 唐玉超 郝春莲 渠雨潇 郝泽慧 徐雷锋[2] 胡凤荣[1] 杨盼盼[2] 明军[2] YIN Xiaoyu;HE Guoren;BI Mengmeng;TANG Yuchao;HAO Chunlian;QU Yuxiao;HAO Zehui;XU Leifeng;HU Fengrong;YANG Panpan;MING Jun(College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037,China;State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Life Sciences,Shanghai Normal University,Shanghai 200233,China;College of Landscape Architecture,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]南京林业大学风景园林学院,南京210037 [2]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081 [3]上海师范大学生命科学院,上海200233 [4]北京农学院园林学院,北京102206

出　　处：《园艺学报》2023年第10期2117-2127,共11页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31902043,32172612);国家重点研发计划项目(2019YFD1001002);中央级公益性科研院所基本科研业务费专项(IVF-BRF2022015)。

摘　　要：以卷丹(Lilium lancifolium)为试材,克隆了1个LBD转录因子基因,命名为LlLBD18(GenBank序列号ON455210)。其开放阅读框为684 bp,编码227个氨基酸,含有1个典型的LOB结构域。进化树分析发现LlLBD18与姜(Zingiber officinale)ZoLBD18的亲缘关系最近。亚细胞定位结果显示LlLBD18定位于细胞质和细胞核。荧光定量PCR分析表明,LlLBD18在卷丹初生珠芽中表达量最高,其次是根中,在叶片中最低。在珠芽形成过程中,LlLBD18的整体表达情况为先上升后下降再上升,且在珠芽原基启动前期(离体诱导4 d)最高。功能研究发现,在卷丹中过表达LlLBD18可以促进珠芽形成,而沉默LlLBD18后的珠芽形成受到显著抑制,表明LlLBD18在卷丹珠芽形成中起正向调节作用。In this study,a LBD transcription factor gene,named LlLBD18(GenBank accession number ON455210)was cloned from Lilium lancifolium.Its open reading frame was 684 bp,which encoded 227 amino acids,and contained a typical LOB domain.The phylogenetic tree analysis showed that LlLBD18 had the highest homology with Zingiber officinale ZoLBD18.Subcellular localization results showed that LlLBD18 was localized in the cytoplasm and nucleus.Quantitative real-time PCR analysis showed that the expression of LlLBD18 was the highest in primary bulbils,followed by roots,and the lowest in leaves.In the process of bulbil formation,the overall expression of LlLBD18 was first increased,then decreased,and then increased,and the highest expression was at the early stage of bulbil primordium initiation(the 4th day).Functional studies found that overexpression of LlLBD18 in L.lancifolium can promote bulbil formation,while the formation of bulbils after VIGS silencing of LlLBD18 was significantly inhibited.This research suggested that LlLBD18 plays a positive regulatory role in bulbil formation of L.lancifolium.

关 键 词：卷丹 侧生器官 珠芽形成 基因克隆 功能分析 

分 类 号：S682.2[农业科学—观赏园艺]