油菜素内酯和寡糖素提高杨梅对凋萎病抗性的机理初探  

Preliminary Study on the Mechanism of Improvement of Resistance to Chinese Bayberry Twig Blight Disease by Brassinolide and Oligosaccharin

作　　者：俞浙萍 严秀娟 张淑文[1] 郑锡良[1] 孙鹂[1] 任海英 梁森苗[1] 戚行江[1,3] YU Zheping;YAN Xiujuan;ZHANG Shuwen;ZHENG Xiliang;SUN Li;REN Haiying;LIANG Senmiao;QI Xingjiang(Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Life Science,China Jiliang University,Hangzhou 310018,China;Xianghu Lab.,Hangzhou 311231,China)

机构地区：[1]浙江省农业科学院园艺研究所,杭州310021 [2]中国计量大学生命科学学院,杭州310018 [3]湘湖实验室,杭州311231

出　　处：《园艺学报》2023年第10期2229-2241,共13页Acta Horticulturae Sinica

基　　金：浙江省重点研发计划项目(2021C02009,2020C02001)。

摘　　要：为探究免疫诱导剂油菜素内酯和寡糖素对杨梅凋萎病的抗性诱导机理,以‘东魁’杨梅幼苗为试材,研究外源诱导剂对接种凋萎病菌后杨梅的抗性、生长指标、叶绿素含量、抗氧化酶活性及病程相关基因表达的影响。结果表明:2,4–表油菜素内酯和寡糖·链蛋白可显著降低杨梅凋萎病病情指数,抗性诱导效果分别达47.94%和39.01%;两者均能有效促进杨梅幼苗生长发育,处理后的杨梅株高、叶长和叶面积均高于对照;在接种后28 d,2,4–表油菜素内酯和寡糖·链蛋白处理显著提高了杨梅叶片叶绿素a、叶绿素b和类胡萝卜素含量,较对照提高13.9%~26.8%。在2,4–表油菜素内酯和寡糖·链蛋白处理下,SOD、POD和CAT酶活性短时间内显著升高,28 d时2,4–表油菜素内酯处理分别比对照提高87.4%、106.5%和77.9%,而MDA含量显著低于对照。2,4–表油菜素内酯和寡糖·链蛋白均能显著诱导杨梅病程相关基因MrPR1、MrPR2和MrPR10上调表达,在接种后7~28 d仍维持在较高表达水平。综上,2,4–表油菜素内酯和寡糖·链蛋白对杨梅的凋萎病抗性诱导效果较好,其诱导机理可能与抗氧化酶活性的增强以及病程相关基因的上调表达密切相关。To explore the mechanism of induced resistance by immune inducers to Chinese bayberry(Myrica rubra)twig blight disease,young plants of‘Dongkui’were used as test materials to study the effects of exogenous inducers on the induction effect,growth index,chlorophyll content,antioxidant enzyme activity and expressions of pathogenesis-related genes in M.rubra after inoculation with twig blight strains.The results showed that 2,4-epibrassinolide(EBR)and oligosaccharins·plant activator protein(OPAP)could significantly reduce the twig blight disease index,with better effects of resistance induction(reaching 47.94%and 39.01%,respectively).Both EBR and OPAP effectively promoted the growth of M.rubra,including plant height,leaf length and leaf area,which were higher than those of the control.Furthermore,EBR and OPAP significantly improved the contents of chlorophyll a,chlorophyll b and carotenoids on 28-day after inoculation,which were increased by 13.9%–26.8%compared with the control.Under the treatment of EBR and OPAP,the activities of SOD,POD and CAT significantly increased in a short time,and their activities were increased by 87.4%,106.5%and 77.9%compared with the control on 28-day after inoculation,while MDA content was lower than that of control.Both EBR and OPAP could significantly induce up-regulated expressions of M.rubra pathogenesis-related genes during 7-day to 28-day after inoculation,including MrPR1,MrPR2 and MrPR10,and their expressions were maintained at a high level.In conclusion,EBR and OPAP have better effects on the induced resistance to M.rubra twig blight disease,and their potential induction mechanisms may be closely related to the enhancement of antioxidant enzyme activity and the up-regulation of pathogenesis-related genes.

关 键 词：杨梅 诱导剂 油菜素内酯 寡糖素 凋萎病 抗氧化酶活性 病程相关蛋白 

分 类 号：S667.6[农业科学—果树学]