中华猕猴桃全基因组MADS-box基因家族鉴定及表达分析  

Genome-wide identification and expression analysis of the MADS-box gene family in Actinidia chinensis

作　　者：高欢 郑珂昕 廖光联 王海令 陈璐 贾东峰 黄春辉[1,2] 曲雪艳[1,2] 徐小彪[1,2] GAO Huan;ZHENG Kexin;LIAO Guanglian;WANG Hailing;CHEN Lu;JIA Dongfeng;HUANG Chunhui;QU Xueyan;XU Xiaobiao(College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China;Kiwifruit Institute of Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China)

机构地区：[1]江西农业大学农学院,南昌330045 [2]江西农业大学猕猴桃研究所,南昌330045

出　　处：《果树学报》2023年第11期2307-2324,共18页Journal of Fruit Science

基　　金：国家自然科学基金项目(32160692)。

摘　　要：【目的】鉴定并分析中华猕猴桃MADS-box基因家族成员,探明MADS-box基因家族成员在果实生长发育及芽休眠中的表达模式。【方法】基于中华猕猴桃红阳V3基因组数据库,利用生物信息学方法对中华猕猴桃MADS-box全基因组进行鉴定,分析其家族成员的理化性质、系统进化树、保守基序、顺式作用元件,并对其在果实生长发育及不同组织中的表达模式进行分析。【结果】在中华猕猴桃红阳基因组中鉴定到了68个AcMADS-box基因,共包含11个亚家族,不均匀地分布于22条染色体上,成员间共存在32对共线性基因对;在基因家族上游启动子区域发现与光响应、激素响应、逆境胁迫响应等相关的顺式元件;17个AcMADS-box基因在组织间高表达且有表达特异性,推测是参与调控中华猕猴桃生长发育的关键基因。【结论】初步鉴定并提供了AcMADS-box家族成员信息,16个AcMADS-box家族成员在根、枝、茎、叶、花中高表达,8个家族成员在花芽中高表达。结果为进一步研究AcMADS-box参与中华猕猴桃的生长发育调控机制提供参考。【Objective】MADS-box transcription factor plays an important role in plant growth and development,such as regulating abiotic stress response,transition from vegetative growth to reproductive growth,root development and fruit maturation.Members of the MADS-box gene family have been identified in several species.However,knowledge about MADS-box gene family members,and their evolutionary and functional characteristics is limited in kiwifruit.The purpose of this study was to identify and analyze MADS-box gene family and determined its expression during the fruit development and bud dormancy,so as to provide theoretical basis for the regulation mechanism of MADS-box gene family members.【Methods】Based on the Hongyang‘V3'genome data of Actinidia chinensis,MADS-box was identified and its gene structure,chromosome distribution,phylogeny,promoter cis-acting element and expression patterns were analyzed.【Results】68 AcMADS-box genes were identified in the Hongyang genome of A.chinensis,among which 35 belonged to Type I genes and 33 belonged to Type Ⅱgenes.There were 11 subfamilies(Mγ,Mβ,Mδ,Mα,FLM,SOC1,ANR1,SVP,AP3,SHP and SEP).AcMADS-box gene was unevenly distributed on 21 chromosomes except 8,10,17,18,19,22 and 24.There were 32 collinear gene pairs,11,4 and 3 pairs of MIKC,M,MIKC and M genomes,respectively.The results of the physical and chemical properties showed that the protein encoded by AcMADS-box gene family was composed of 77 to 1703 aa,the molecular weight of the protein was 8 917.01 to121 149.91 u,and the isoelectric point was 4.55 to 11.25.Except AcMIDS24-MIKC,the protein was fatsoluble hydrophilic protein.AcMADS24-MIKC was a water-soluble hydrophilic protein.The CDS sequence length encoded by AcMADS-box protein was 234 to 1791 bp except for AcMIDS33-M,and the CDS sequence length encoded by AcMIDS33-M gene was 3222 bp,which was significantly higher than other genes of AcMADS-box gene family.In addition,the subcellular localization prediction analysis showed that the AcMADS-box gene family member

关 键 词：中华猕猴桃 MADS-BOX 光响应 顺式元件 生长发育 

分 类 号：S663.4[农业科学—果树学]