葡萄资源叶片抗炭疽病鉴定及抗病基因的QTL定位  

Identification of resistance to anthracnose in leaves of grape resources and QTL localization of disease resistance genes

作　　者：禹方方 张颖[1] 姜建福[1] 孙磊[1] 刘崇怀[1] 樊秀彩[1] YU Fangfang;ZHANG Ying;JIANG Jianfu;SUN Lei;LIU Chonghuai;FAN Xiucai(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,Henan,China)

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009

出　　处：《果树学报》2023年第11期2325-2339,共15页Journal of Fruit Science

基　　金：国家园艺种质资源库(NHGRC2022-NH00-2);国家葡萄农业产业技术体系(CARS-29-yc-1);中国农业科学院科技创新工程专项(CAAS-ASTIP-2022-ZFRI)。

摘　　要：【目的】炭疽病是葡萄的主要病害之一,极易造成大规模的病害流行,严重影响葡萄产量及品质,筛选抗病种质及挖掘抗病基因有利于葡萄抗病机制研究及抗病育种的开展。【方法】利用室内离体叶片接种法,对60份中国野生葡萄种质、122份欧亚种质、76份欧美杂种以及美人指×刺葡萄0940的F1代杂交群体进行炭疽病抗性鉴定与评价,并利用SNP标记构建的遗传图谱对炭疽病抗性进行QTL定位。【结果】经抗性鉴定,共筛选出1份高抗、43份抗病和75份中抗种质,分别占总鉴定种质的0.39%、16.67%和29.07%。以筛选出的抗病种质刺葡萄0940和感病种质美人指的杂交后代为分离群体进行QTL定位,在第8号连锁群上检测到一个与抗炭疽病相关的QTL位点,可解释14.7%的表型变异。根据QTL定位区间基因注释结果,筛选出15个抗病相关基因,推测它们在葡萄抗炭疽病中发挥一定作用。【结论】明确了不同葡萄种质的炭疽病抗性水平,定位到1个抗炭疽病QTL位点并筛选出15个抗病相关基因。【Objective】Grape anthracnose is one of the main diseases of grape,which mainly infects the fruits,young leaves and new branches of grape,causing fruit rot,shedding,or water loss and shrinkage into stiff fruits.Breeding disease-resistant grape varieties is the most economical,effective,and environmentally friendly long-term control strategy for the prevention of the anthracnose disease.There have been a few studies on the identification of the resistance to anthracnose and the QTL localization of relative disease resistant genes so far.The study aimed to identify the resistance to the anthracnose disease of different grape germplasms and seek for new QTL locus for the resistance and provide germplasm materials and basis for the breeding and research on the disease resistance mechanism.【Methods】The indoor ex vivo leaf inoculation method was used to identify and evaluate the resistance to anthracnose in 60 strains of Chinese wild grapes,122 accessions of V.vinifera and 76 accessions of V.vinifera-V.labrusca,and the hybrid offsprings of Manicure Finger(susceptible)×Ciputao0940(resistant),and the QTL localization of grape anthracnose resistance was carried out using the genetic map constructed by SNP markers.【Results】A total of 1 germplasm with high resistance,43 germplasms with resistance and 75 germplasms with medium resistance were screened out,accounting for 0.39%,16.67%and 29.07%of the total identified accessions,respectively.There were great differences in the resistance to anthracnose in different germplasm populations.63.34%of the East Asian population56.57%of the V.vinifera-V.labrusca population,and 31.15%of the Eurasian population had the resistance to anthracnose.In the identification of anthracnose resistance in Chinese wild grape germplasms,it was found that the resistance of different strains in the same population varied greatly,and the species with strong resistance on the whole included V.davidii,V.pseudoreticulata,V.amurensis,and V.piasezkii.The strong resistance of the strains include S

关 键 词：葡萄炭疽病 离体叶片接种鉴定 QTL定位 候选基因 

分 类 号：S663.1[农业科学—果树学]