玉米果穗苞叶包裹度的QTL分析  

QTL Analysis of Husk Coverage on Maize(Zea mays)Ear

作　　者：朱秋丽 张舒钰 章慧敏 宋旭东 张振良[1] 陆虎华[1] 陈国清[1,3] 郝德荣 冒宇翔[1] 石明亮[1] 薛林[1,3] 周广飞[1] ZHU Qiu-Li;ZHANG Shu-Yu;ZHANG Hui-Min;SONG Xu-Dong;ZHANG Zhen-Liang;LU Hu-Hua;CHEN Guo-Qing;HAO De-Rong;MAO Yu-Xiang;SHI Ming-Liang;XUE Lin;ZHOU Guang-Fei(Jiangsu Yanjiang Institute of Agricultural Science,Nantong 226012,China;Nantong Crop Cultivation Technique Direction Station,Nantong 226007,China;Jiangsu Collaborative Innovation Centre for Modern Crop Production,Nanjing 210095,China)

机构地区：[1]江苏沿江地区农业科学研究所,南通226012 [2]南通市作物栽培技术指导站,南通226007 [3]江苏省现代作物生产协同创新中心,南京210095

出　　处：《农业生物技术学报》2023年第11期2231-2238,共8页Journal of Agricultural Biotechnology

基　　金：江苏省种业振兴揭榜挂帅项目(JBGS[2021]054);江苏现代农业产业技术体系建设专项资金(JATS[2022]150);南通市科技局项目(MS22021039;JC2021153);江苏沿江地区农业科学研究所学科建设专项基金(YJXK[2021]201)。

摘　　要：玉米(Zea mays)苞叶包裹度不仅与玉米穗腐病抗性密切相关,而且也是影响玉米机械直收籽粒的重要性状之一,鉴定控制苞叶包裹度的遗传位点,有助于目标性状的遗传改良。本研究以衍生于T877×DH4866的204个重组自交系为材料,在5个环境下鉴定田间表型,使用Axiom?Maize56K SNP Array开展基因型分析。单个环境QTL分析共检测到9个控制苞叶包裹度QTL,单个QTL可解释4.70%~17.00%的表型变异,其中,8个QTL是新的遗传位点。多个环境QTL分析共检测到26个QTL与环境互作,单位点的贡献率为0.73%~4.77%,与环境互作的贡献率为0.10%~4.55%。qHC4.09是1个稳定的玉米苞叶包裹度QTL,与玉米黄曲霉菌(Aspergillus flavus)和禾谷镰孢菌(Fusarium graminearum)穗腐病抗性QTL区间重合,可能是1个控制玉米苞叶包裹度和穗腐病抗性的多效性位点。本研究为玉米苞叶包裹度的遗传基础解析和分子标记辅助改良提供参考。Maize(Zea mays)husk coverage is associated with the resistance to Aspergillus and Gibberella ear rot,also affects the mechanical harvesting of maize grain.Thus,the identification of genetic loci for husk coverage will provide available targets for maize molecular breeding.In this study,a set of 204 recombinant inbred lines developed from the T877×DH4866,was phenotypically evaluated under 5 field environments,and was genotyped using Axiom®Maize56K SNP Array.A total of 9 QTL for husk coverage were detected by individual environmental QTL mapping,of which 8 QTLs were putatively novel loci,the phenotypic variance explained by single QTL ranged from 4.70%to 17.00%.In addition,26 additive QTL-by-environment loci were detected by joint environmental QTL analysis.The phenotypic variance explained by single additive QTL ranged from 0.73%to 4.77%,and the contributions of interaction between each additive QTL and environment ranged from 0.10%to 4.55%.qHC4.09,a stable QTL for husk coverage,was colocalized with the genomic region for maize resistance to Aspergillus and Gibberella ear rot,suggesting that this locus might be a pleiotropic QTL for maize husk coverage and resistance to ear rot.These results could provide theoretical basis for elucidating the genetic basis of husk coverage and available marker for maize molecular breeding.

关 键 词：玉米 苞叶包裹度 QTL 

分 类 号：S513[农业科学—作物学]