织金白鹅微卫星标记遗传多样性及其与体尺指标的关联分析  

Genetic Diversity of Microsatellite Markers in Zhijin White Geese(Anser cygnoides orientalis)and Its Association Analysis with Body Size Indexes

作　　者：郭徵力 赵中龙 杨红 王天松 叶丽 周艳 杨远青 袁建 王智伟 张勇[1,4] GUO Zhi-Li;ZHAO Zhong-Long;YANG Hong;WANG Tian-Song;YE Li;ZHOU Yan;YANG Yuan-Qing;YUAN Jian;WANG Zhi-Wei;ZHANG Yong(Key Laboratory of Genetic Breeding and Reproduction of Plateau Mountain Animals,Ministry of Education/Key Laboratory of Animal Genetic Breeding and Reproduction/College of Animal Science,Guizhou University,Guiyang 550025,China;Bijie City Animal Husbandry Station,Bijie 551700,China;Agricultural college,Tongren Polytechnic College,Tongren 554300,China;Guizhou Cattle Industry Group Science&Technology Services Co.,Ltd.,Guiyang 550025,China)

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖教育部重点实验室/贵州省动物遗传育种与繁殖重点实验室/动物科学学院,贵阳550025 [2]毕节市畜牧站,毕节551700 [3]铜仁职业技术学院农学院,铜仁554300 [4]贵州黄牛产业集团科技服务公司,贵阳550025

出　　处：《农业生物技术学报》2023年第11期2341-2357,共17页Journal of Agricultural Biotechnology

基　　金：贵州省科技计划项目(黔科合支撑[2020]1Y047号;黔科合支撑[2021]一般156)。

摘　　要：织金白鹅(Anser cygnoides orientalis)是经长期人工选择和风土驯化形成的地方品种,具有耐粗饲、适应性强等特点。为探究织金白鹅准确的遗传信息,筛选与其体尺性状相关的位点,本研究利用微卫星技术分析织金白鹅群体内遗传多样性,并在与体尺相关的位点中进行不同基因型的多重比较,旨在为织金白鹅的种质资源保护及开发利用提供一定的理论基础。研究共测量了101羽织金白鹅体尺指标,从52对微卫星引物中筛选出15对多态性较好的微卫星引物,共检测出63个等位基因,平均有效等位基因数为2.0665,其中以CKW21位点有效等位基因数最多。各位点观测杂合度范围在0~0.9697之间,期望杂合度范围在0.2657~0.6762之间,Ans17位点期望杂合度值最低,CKW21位点期望杂合度值最高。香农指数处于0.5392~1.3074之间,CKW21位点的香农指数值最高。各位点多态信息含量范围在0.2489~0.6202之间,Ans17位点的多态信息含量值最低,CKW21位点的多态信息含量最高。近交系数平均值为0.1972。各位点经过卡方检验发现位点5A5397、5A265164、Ans21、Ans25、CKW10、CKW48、TTUCG2极显著偏离哈德-温伯格平衡。相关性分析发现,在公鹅中,有5个位点分别与体重、体斜长、胸宽、龙骨长、骨盆宽、胫长相关;在母鹅中,有10个位点分别与体重、胸宽、胸深、龙骨长、骨盆宽、胫长、胫围及半潜水长存在不同程度的相关。研究结果为织金白鹅分子标记辅助育种提供一定参考。The Zhijin white goose(Anser cygnoides orientalis)is a local breed developed through long-term artificial selection and terroir domestication,and it possesses the traits of tough feeding resistance and great adaptability.To investigate the accurate genetic information and to screen the loci associated with body size traits in Zhijin white goose,in this study,microsatellite technology was used to analyze the genetic diversity within the Zhijin white goose population and performed multiple comparisons of different genotypes in loci related to body size,aiming to provide a theoretical basis for the conservation and development of germplasm resources of Zhijin white goose.A total of 101 Zhijin white geese were measured,and 15 pairs of microsatellite primers with good polymorphism were selected from 52 pairs of microsatellite primers.There were 63 alleles detected;The average number of valid alleles was 2.0665;The CKW21 locus contained the most valid alleles.The observed heterozygosity of each locus ranged from 0 to 0.9697 and the expected heterozygosity ranged from 0.2657 to 0.6762,with the lowest expected heterozygosity value for locus Ans17 and the highest expected heterozygosity value for locus CKW21.The Shannon index ranged from 0.5392 to 1.3074,and the CKW21 locus had the highest Shannon's information index.The polymorphism information content of each locus ranged from 0.2489 to 0.6202,with the Ans17 locus having the lowest polymorphism information content and the CKW21 locus having the highest polymorphism information content.The mean value of the inbreeding coefficient was 0.1972.The loci 5A5397,5A265164,Ans21,Ans25,CKW10,CKW48,and TTUCG2 were found to deviate from Hardy-Weinberg equilibrium significantly by chi-square test.The correlation analysis revealed that in male geese,5 loci were correlated with body weight,body oblique length,chest width,sternum length,pelvic width and shank length,respectively;In female geese,10 loci were correlated with different body size indexes except body oblique length.The resu

关 键 词：织金白鹅 微卫星DNA标记 体尺性状 遗传多样性 

分 类 号：S835[农业科学—畜牧学]