蛋内注射缬氨酸对鸭胚生长发育和肝脏脂肪沉积的影响  

Effects of in ovo Injection of Valine on the Growth and Development of Duck(Anas platyrhynchos)Embryo and Hepatic Fat Deposition

作　　者：张昱 厐彦芹 张镕 王立 王家乡[1] 李鹏[1] 杨彩侠 杜志强 ZHANG Yu;PANG Yan-Qin;ZHANG Rong;WANG Li;WANG Jia-Xiang;LI Peng;YANG Cai-Xia;DU Zhi-Qiang(College of Animal Science,Yangtze University,Jingzhou 434025,China)

机构地区：[1]长江大学动物科学学院,荆州434025

出　　处：《农业生物技术学报》2023年第11期2358-2366,共9页Journal of Agricultural Biotechnology

基　　金：安徽省畜禽品种联合育种项目(2021-2025)。

摘　　要：支链氨基酸(branched chain amino acids,BCAAs)包括亮氨酸、异亮氨酸和缬氨酸,影响家禽产业的生产效率和产品质量。为探寻BCAAs失衡阻碍家禽的生长发育的分子机制,本研究通过胚蛋注射技术建立鸭(Anas platyrhynchos)胚蛋内缬氨酸喂养模型,对其表型和孵化时间进行分析,进一步采用ELISA、石蜡切片和qPCR等技术探讨缬氨酸处理对鸭胚肝脏脂肪沉积的影响。结果表明,2 mg/100μL缬氨酸处理显著缩短孵化时间,显著改变雏鸭的胸肉重量、雄性胰腺重量和雌性性腺(卵巢)长度,以及不同发育阶段胚胎血清中的胰岛素样生长因子1(insulin like growth factor 1,IGF-1)水平(P<0.05)。肝脏石蜡切片显示,处理组脂滴数量显著增加(P<0.05)。IGF-1途径和脂肪生成相关基因的表达水平也发生显著变化(P<0.05),且具有性别特异性。综上,鸭胚缬氨酸处理可以影响其生长发育,促进肝脏脂肪合成。本研究为BCAAs失衡影响家禽生产提供了新的研究视角和材料。Branched chain amino acids(BCAAs)including leucine,isoleucine and valine,affect the production efficiency and product quality of poultry industry.In order to further explore the molecular mechanism of BCAAs imbalance hindering the growth and development of poultry,this study established a duck(Anas platyrhynchos)embryo egg feeding model by embryo egg injection technology,analyzed its phenotype and incubation time.Furthermore,ELISA,paraffin section and qPCR techniques were used to examine the effect of valine on liver fat deposition in duck embryos.The results showed that valine treatment significantly shortened the incubation time,significantly changed the breast weight,male pancreas weight and female gonad(ovary)length of ducklings,as well as the level of insulin like growth factor 1(IGF-1)in the serum of embryos at different stages of development(P<0.05).The number of lipid droplets in the treatment group increased significantly.The expression level of IGF-1 pathway and adipogenesis related genes also changed significantly(P<0.05)and had sex specificity.To sum up,duck embryo valine treatment can affect its growth and development,promote liver fat synthesis.This study provides a new research perspective and material for the impact of BCAAs imbalance on poultry production.

关 键 词：蛋内注射 缬氨酸 鸭胚 肝脏 脂肪沉积 

分 类 号：S834.8[农业科学—畜牧学]