西门塔尔牛9种遗传缺陷KASP检测方法的建立  

Development of KASP Assays of 9 Genetic Defects in Simmental Cattle(Bos taurus)

作　　者：裴芬 张可 KHAN Md Yousuf Ali 司敬方 代冬梅 欧四海[2] 杨阳[2] 赵宗胜[3] 王雅春[1] 张毅[1] PEI Fen;ZHANG Ke;KHAN Md Yousuf Ali;SI Jing-Fang;DAI Dong-Mei;OU Si-Hai;YANG Yang;ZHAO Zong-Sheng;WANG Ya-Chun;ZHANG Yi(College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;Shihezi Animal Husbandry and Veterinary Workstation,The Eighth Division of Xinjiang Production and Construction Corps,Shihezi 832000,China;College of Animal Science and Technology,Shihezi University,Shihezi 832000,China)

机构地区：[1]中国农业大学动物科技学院,北京100193 [2]新疆生产建设兵团第八师石河子市畜牧兽医工作站,石河子832000 [3]石河子大学动物科技学院,石河子832000

出　　处：《农业生物技术学报》2023年第11期2435-2442,共8页Journal of Agricultural Biotechnology

基　　金：新疆生产建设兵团科技计划(2021AA004);财政部和农业农村部:国家现代农业产业技术体系(CARS36)。

摘　　要：西门塔尔牛(Bos taurus)及其相关品种是我国乳肉兼用牛和肉用牛养殖的主要品种。近年来,国内外学者利用基因组技术在西门塔尔牛群体中鉴定到了多种遗传缺陷,这些遗传缺陷会导致胚胎早期死亡、新生犊牛缺陷、生殖力下降等问题,给牧场带来经济损失。本研究利用竞争性等位基因特异性PCR(kompetitive allele-specific PCR,KASP)分型技术,设计特异性引物,对侏儒症、蜘蛛腿综合征、并趾症、血小板病、锌缺乏样综合征、公牛生育力低下、瑞士褐牛单倍型2、弗莱维赫牛单倍型2和弗莱维赫单倍型4共9种西门塔尔牛遗传缺陷实现分型,96个测试样本的分型检出率达100%,共检测出7种遗传缺陷的携带者。本研究为我国种牛遗传物质进口、种公牛培育和母牛选种选配过程中有害基因的筛查和风险管理提供了技术手段。Simmental cattle(Bos taurus)and its related breeds are the main dual-purpose breeds and beef breeds in China.In recent years,multiple genetic defects,causing early embryonic death,newborn calf defects,reduced fertility and other problems and resulting in economic losses to farms,have been identified in Simmental cattle populations using genomic technologies.This study aimed to develop an accurate and efficient method based on kompetitive allele-specific PCR(KASP)assays to screen 9 genetic defects in Simmental cattle,including Dwarfism,Arachnomelia syndrome,Syndactyly,Thrombopathy,Zinc deficiencylike disorders,Bovine male subfertility,Brown Swiss haplotype 2,Fleckvieh haplotype 2 and Fleckvieh haplotype 4.The call rate was 100%for the 96 tested samples,and carriers were detected on 7 genetic defects.This study provides a technical tool for screening and management of harmful genes during the importation of genetic materials,selection of sires and mating programs of cows in China.

关 键 词：竞争性等位基因特异性PCR(KASP) 遗传缺陷 西门塔尔牛 分子检测 

分 类 号：S823[农业科学—畜牧学]