过岗龙的化学成分研究  

Study on chemical constituents from stems of Entada phaseoloides

作　　者：胡乐诗 谭少丽 朱子云 刘中秋 吴爱芝[1] 赵钟祥[1] 杨伟群 HU Le-shi;TAN Shao-li;ZHU Zi-yun;LIU Zhong-qiu;WU Ai-zhi;ZHAO Zhong-xiang;YANG Wei-qun(School of Pharmaceutical Science,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Department of Pharmacy,the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510400,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学第一附属医院药学部,广东广州510400

出　　处：《中草药》2023年第20期6596-6602,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(82104539);广东省教育厅青年创新人才项目(2021KQNCX012)。

摘　　要：目的研究过岗龙(榼藤子Entada phaseoloides干燥藤茎)的化学成分。方法应用正相硅胶、ODS、Sephadex LH-20凝胶柱色谱以及半制备型HPLC等色谱技术进行系统分离纯化,并通过核磁共振波谱、质谱等技术鉴定单体化合物的结构。结果从过岗龙75%乙醇提取物中共分离得到17个化合物,分别鉴定为3-氧-泰国树脂酸(1)、6β-羟基-3-氧代齐墩果-12-烯-28酸(2)、泰国树脂酸(3)、苏门答腊树脂酸(4)、3β,6β-二羟基齐墩果-11,13(18)-二烯-28-酸(5)、(Z)-4-[3′-(β-Dglucopyranosyloxy)butylidene]-3,5,5-trimethyl-2-cyclohexen-l-one(6)、异落新妇苷(7)、黄杞苷(8)、槲皮苷(9)、3-O-α-L鼠李糖-儿茶素(10)、(−)-epicatechin-3-O-p-hydroxybenzoate(11)、lysidicichin(12)、异补骨脂查耳酮(13)、4-羟基-3-甲氧基苯酚-1-O-β-D-(6′-O-没食子酰基)葡萄糖苷(14)、1,6-二-O-没食子酰基-β-D-葡萄吡喃糖(15)、苯甲酸(16)和没食子酸甲酯(17)。结论所有化合物均为首次从过岗龙中分离得到,为中药过岗龙的开发利用提供了一定的物质基础。Objective To study the chemical constituents from the stems of Entada phaseoloides.Methods The chemical constituents were isolated and purified by silica gel,ODS,Sephadex LH-20 gel column chromatography and semi-preparation HPLC.The structures of all isolates were determined on the basis of NMR and MS analysis.Results A total of 17 compounds were isolated and identified as 3-oxo-siaresinolic acid(1),6β-hydroxy-3-oxo-olean-12-en-28-oic acid(2),siaresinolic acid(3),sumaresinolic acid(4),3β,6β-dihydroxy olean-11,13(18)-dien-28-acid(5),(Z)-4-[3′-(β-D-glucopyranosyloxy)butylidene]-3,5,5-trimethyl-2-cyclohexen-l-one(6),isoastibin(7),engeletin(8),quercitrin(9),catechin-3-O-α-L-rhamnopyranoside(10),(−)-epicatechin-3-O-p-hydroxybenzoate(11),lysidicichin(12),isobavachalcone(13),4-hydroxy-3-methoxy-phenol-1-O-β-D-(6′-O-galoyl)glucopyranoside(14),1,6-di-O-galloyl-β-D-glucopyranoside(15),benzoic acid(16),and gallic acid methlyl ester(17).Conclusion All compounds are isolated from the stems of E.phaseoloides for the first time.This study provides certain study basis for the utilization of E.phaseoloides.

关 键 词：过岗龙 三萜 降倍半萜苷 黄酮类 酚苷 6β-羟基-3-氧代齐墩果-12-烯-28酸 异落新妇苷 黄杞苷 槲皮苷 

分 类 号：R284.1[医药卫生—中药学]