欧耧斗菜AP2/ERF基因家族鉴定及盐胁迫下表达分析  

Identification of AP2/ERF gene family in Aquilegia vulgarisand expression analysis under salt stress

作　　者：王玉凤 孟缘 于海航 崔丁元 白云 WANG Yufeng;MENG Yuan;YU Haihang;CUI Dingyuan;BAI Yun(College of Forestry and Grassland,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学林学与草学学院,长春130118

出　　处：《广西植物》2023年第10期1861-1875,共15页Guihaia

基　　金：国家自然科学基金(32101586);吉林省自然科学基金(YDZJ202201ZYTS452)。

摘　　要：AP2/ERF转录因子在植物生长发育和响应非生物胁迫中发挥着重要作用。为探究欧耧斗菜(Aquilegia vulgaris)中AP2/ERF对盐胁迫的响应,该研究基于前期试验获得的盐胁迫下转录组数据,通过生物信息学方法筛选欧耧斗菜AP2/ERF家族基因,分析其生化特征、保守基序、系统进化等,并对其在盐胁迫处理下不同时间的根与叶中的表达量变化进行分析,利用qRT-PCR技术对候选基因表达量进行验证。结果表明:(1)筛选出86个AvAP2/ERF基因,其编码的氨基数目为132~722个,分子量为14 763.30~79 069.47 Da,等电点介于4.49~9.68之间,偏酸性蛋白较多,均为亲水性蛋白;对AvAP2/ERF蛋白进行亚细胞定位预测,大多数定位于细胞核。(2)二级结构以无规则卷曲和α-螺旋为主,均具有AP2保守结构域,有两个高度保守的基序Motif 1和Motif 2。(3)在盐胁迫下,71个AvAP2/ERF基因表达量发生变化,其中叶片中差异表达基因18个、根中19个;欧耧斗菜与拟南芥AP2/ERF基因聚类为5个亚家族、15个亚组,通过表达分析及同源关系,确定3个响应盐胁迫的基因AvAP2/ERF-56、AvAP2/ERF-61与AvAP2/ERF-80,其qRT-PCR结果与转录组数据一致。该研究结果为深入探究欧耧斗菜AP2/ERF基因的功能及逆境响应机制奠定了基础。AP2/ERF transcription factors play important roles in plant growth,development and response to abiotic stress.In order to explore the response of AvAP2/ERF genes in Aquilegia vulgaris to salt stress,based on the transcriptome data obtained under salt stress in previous experiments,the AP2/ERF gene family in A.vulgaris were screened by bioinformatic methods,and their physical and chemical properties,conserved motifs,phylogenetic relations and expression changes of these genes in leaves and roots under salt stress were analyzed,etc.The expression of candidate genes was verified by qRT-PCR.The results were as follows:(1)86 AvAP2/ERF genes were identified which encoded 132-722 amino acids,with molecular weight of 14763.30-79069.47 Da and isoelectric point ranged from 4.49 to 9.68.Most of them were slightly acidic proteins and all of them were hydrophilic.Most of AvAP2/ERF were localized in nucleus.(2)The similarity of secondary structure was high,which was proportionally composed of random coil andα-helix.The members all contained AP2 domains,and two conserved motifs were predicted.(3)Under different stages of salt treatment,there were 71 AvAP2/ERF genes responded to salt stress.There were 18 and 19 differentially expressed genes in leaves and roots,respectively.There were 86 AP2/ERF genes of A.vulgaris were divided into five subfamilies clustering with A.thaliana;the AP2/ERF genes of A.vulgaris and Arabidopsis thaliana were clustered into five subfamilies and 15 subgroups.Through expression analysis and homology relationship,AvAP2/ERF-56,AvAP2/ERF-61 and AvAP2/ERF-80 of them might be involved in salt resistance,and the qRT-PCR results were consistent with sequencing expression trends.The results of this study provides a reliable reference for further research on the function and stress response mechanism of AP2/ERF gene in Aquilegia vulgaris.

关 键 词：欧耧斗菜 AP2/ERF 生物信息学 盐胁迫转录组 表达分析 

分 类 号：Q943[生物学—植物学]