急性心肌梗死小鼠模型的血清代谢特征:基于LC-MS/MS的靶向代谢组学分析  

Serum metabolic profile in acute myocardial infarction mice model:An LC-MS/MSbased targeted metabolomic analysis

作　　者：寻斯琪 干昌平[1,2] 郭应强[1,2] 秦超毅[1,2] XUN Siqi;GAN Changping;GUO Yingqiang;QIN Chaoyi(Department of Cardiovascular Surgery,West China Hospital,Sichuan University,Chengdu,610041,P.R.China;Cardiovascular Surgery Research Laboratory,West China Hospital,Sichuan University,Chengdu,610041,P.R.China)

机构地区：[1]四川大学华西医院心脏大血管外科,成都610041 [2]四川大学华西医院心脏大血管外科研究室,成都610041

出　　处：《中国胸心血管外科临床杂志》2023年第11期1609-1617,共9页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金青年基金项目(81900311);四川省科技厅基金(2022YFS0364)。

摘　　要：目的利用代谢组学分析心肌梗死(myocardial infarction,MI)的代谢特征,了解其发病机制,并对MI的治疗方向进行新探索。方法超高效液相色谱-三重四极杆串联质谱(UHPLC-QqQ/MS)对10只MI小鼠和5只假手术对照组小鼠的血清代谢物进行分析,并使用SPSS进行统计分析。利用MetaboAnalyst5.0对差异代谢物进行代谢途径分析,构建代谢网络。结果UHPLC-QqQ/MS检测到129种代谢物。MI小鼠与假手术对照组之间存在显著的血清代谢物差异。血清中129种代谢物中的50种与心肌缺血相关。此外,MI最重要的代谢途径是丙氨酸、天冬氨酸和谷氨酸代谢,甘氨酸、丝氨酸和苏氨酸代谢,乙醛酸盐和二羧酸盐代谢。结论在MI中丝氨酸相关代谢途径中的代谢物在血清中含量降低,对MI时心肌保护提出新的治疗方向。Objective To analyze the metabolic characteristics of myocardial infarction(MI)using metabolomics to better understand its pathogenesis and to explore new therapeutic directions for MI.Methods Serum metabolites in ten acute MI mice and five sham-control mice were analyzed by UHPLC-QqQ/MS,and SPSS was used for statistical analysis.MetaboAnalyst 5.0 was used to analyze the metabolic pathways of the differential metabolites and build a metabolic network.Results One hundred and twenty-nine metabolites were detected by UHPLC-QqQ/MS.Significant serum metabolite differences were found between MI mice and normal controls.Fifty out of 129 metabolites in serum were associated with MI.In addition,the most important metabolic pathways were D-glutamate metabolism,alanine,aspartate and glutamate metabolism,glycine,serine and threonine metabolism,glyoxylate and dicarboxylate acid metabolism.Conclusion Metabolites in serine-related metabolic pathways reduce in serum in MI.We propose a new therapeutic direction for myocardial protection in MI.

关 键 词：代谢组学 心肌梗死 丝氨酸 

分 类 号：R542.22[医药卫生—心血管疾病] R-332[医药卫生—内科学]