地塞米松诱导肉鸡脂肪肝模型的构建及效果分析  

Fatty Liver Model Construction and Its Effectiveness Evaluation Induced by Dexamethasone in Broilers

作　　者：王超慧 孙喜 王强刚 刘芮兵 李妮 杨小军[1] 刘艳利[1] WANG ChaoHui;SUN Xi;WANG QiangGang;LIU RuiBing;LI Ni;YANG XiaoJun;LIU YanLi(College of Animal Science and Technology,Northwest A&F University,Yangling 712100,Shaanxi)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国农业科学》2023年第20期4115-4124,共10页Scientia Agricultura Sinica

基　　金：国家重点研发青年科学家项目(2022YFF1001000);陕西省青年科技新星项目(2022KJXX-13);大学生创新创业训练计划(202210712104,S202210712483)。

摘　　要：【背景】腹脂沉积过多和脂肪肝综合征是家禽养殖中面临的重要脂代谢紊乱产业问题,肝脏是家禽体内从头合成脂肪酸的主要部位,也在物质代谢、胆汁生成、解毒等的调节中发挥重要作用。因此解析脂代谢紊乱机理将为家禽产业的健康发展提供参考依据,但畜禽动物发病机理的探究往往需要模型为前提。【目的】通过试验评价地塞米松诱导肉鸡脂肪肝的效果,为家禽脂代谢紊乱相关模型的构建提供参考。【方法】选用体重相近的35日龄AA肉鸡16只,随机分为对照组和地塞米松组,每组8个重复,地塞米松组连续7 d颈部皮下注射地塞米松磷酸钠溶液(4.5 mg·kg-1体重),对照组注射同体积的生理盐水,7 d后观测肉鸡肝脏病理性变化并对其进行油红O染色,同时检测血清生化与机体抗氧化指标,采用实时荧光定量PCR方法检测肝脏中与脂质代谢和炎症反应相关的基因表达。【结果】与对照组相比,地塞米松组肉鸡肝脏指数显著提高(P<0.05),油红O切片显示肉鸡肝组织有大量红色脂滴出现,且肝脏中TG和TC含量均高于对照组(P<0.05)。血清生化结果显示地塞米松组谷草转氨酶(AST)、总胆红素(TBIL)、尿酸(UA)、总蛋白(TP)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)、总胆固醇(TC)和甘油三酯(TG)水平显著高于对照组(P<0.05),但谷丙转氨酶(ALT)、葡萄糖(GLU)含量无显著变化(P>0.05)。地塞米松注射显著提高了肉鸡肝脏ACC、FAS、SCD1、PPARγ、ChREBP、SREBP-1c、IGF2、GR等脂质合成相关基因的表达(P<0.05),但ELOVL6基因的表达量无显著变化(P>0.05);降低了脂质分解代谢相关基因CPT1、LPL、PPARα的mRNA丰度(P<0.05);但地塞米松组与脂质水解相关的ATGL基因以及与脂质转运相关CETP、MTTP基因表达量显著高于对照组(P<0.05);除此之外,地塞米松注射显著提高了肉鸡肝脏MyD88、NFκB、IL-6、TNF-α等与炎症发生相关�【Background】Excessive abdominal fat deposition and fatty liver syndrome are important industrial problems of lipid metabolism disorder in poultry breeding.The liver is the main site of de novo fatty acid synthesis in poultry,which plays a vital function in nutrients metabolism,formation of bile acids and detoxication.Therefore,the mechanism analysis of lipid metabolism disorder will provide reference for the healthy development of poultry industry,but the disease model is necessary for pathogenesis research.【Objective】This experiment was conducted to evaluate the effect of dexamethasone-induced fatty liver in broilers,aiming to provide the reference for the construction of lipid metabolism disorder model in poultry.【Method】Sixteen 35-day-old AA broilers with similar body weight were randomly divided into control group and dexamethasone group(DXM),with 8 replicates in each group.The birds in the DXM were injected subcutaneously with dexamethasone sodium phosphate(4.5 mg·kg-1)for 7 days,and the birds in the control group were given the administration of normal saline injection.After 7 days,oil red O staining was used to analyze the histopathological changes of the liver,and serum biochemical and antioxidant indexes were detected.RT-PCR was applied to detect genes expression related to lipid metabolism and inflammatory response in the liver.【Result】Compared with the control group,the liver index of broilers in the DXM was significantly increased(P<0.05).Oil red O sections showed a large number of red lipid droplets,and the contents of TG and TC in the liver were higher than those in the control group(P<0.05).Serum biochemical results showed that aspartate amino transferase(AST),total bilirubin(TBIL),uric acid(UA),total protein(TP),high density lipoprotein cholesterol(HDL-c),low density lipoprotein cholesterol(LDL-c),total cholesterol(TC)and triglyceride(TG)levels in the DXM were significantly higher than those in the control group(P<0.05),but there was no significant difference about alanine aminotr

关 键 词：地塞米松 肉鸡 脂肪肝综合征 

分 类 号：S858.31[农业科学—临床兽医学]