萧氏松茎象病原真菌的调查与鉴定  

Investigation and Identification of the Entomopathogenic Fungi of Hylobitelus xiaoi Zhang

作　　者：吴跃开 刘童童 李继业 WU Yue-kai;LIU Tong-tong;LI Ji-ye(Guizhou Academy of Forestry,Guiyang,Guizhou 550005;Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550025)

机构地区：[1]贵州省林业科学研究院,贵州贵阳550005 [2]贵州省农业科学院,贵州贵阳550025

出　　处：《安徽农业科学》2023年第21期148-152,169,共6页Journal of Anhui Agricultural Sciences

基　　金：贵州省林业局科技计划项目“萧氏松茎象主要病原真菌应用技术研究”(黔林科合[2018]02号);贵州省科技支撑计划项目“危险性林业有害生物萧氏松茎象的防控技术研究”(黔科合支撑[2019]2309)。

摘　　要：通过野外调查,结合土壤人工诱集技术,共采集到萧氏松茎象的病原真菌12个菌种(菌株)。通过形态特征观测,结合rDNA ITS序列测定比对,5个菌株(ZR100、ZR168、ZR345、ZR349、YJ005)被鉴定为拟球孢白僵菌(Beauveria pseudobassiana),1个菌株(ZR225)被鉴定为球孢白僵菌(B.bassiana),5个菌株(ZR208、YJ001、YJ002、YJ003、YJ004)被鉴定为绿僵菌(Metarhizium sp.),1个菌株(ZR093)被鉴定为轮枝菌(Lecanicillium sp.)。室内感染试验表明,5个拟球孢白僵菌菌株和1个球孢白僵菌菌株对萧氏松茎象成虫及幼虫均表现出明显的致病性,其中拟球孢白僵菌菌株ZR100的致病速度及最终致病率明显更高,说明其致病性最强,可作为萧氏松茎象的首选生防菌种。该研究结果为萧氏松茎象的生物防治提供了重要的物质基础和科学依据。By ways of field investigation and artificial soil bait technique,a total of 12 species(strains)of entomopathogenic fungi were obtained.According to the results of morphological study combing with the rDNA ITS sequence comparisons,5 strains(ZR100,ZR168,ZR345,ZR349,YJ005)were identified as Beauveria pseudobassiana,1 strain(ZR225)as B.bassiana,5 strains(ZR208,YJ001,YJ002,YJ003,YJ004)as Metarhizium sp.,and 1 strain as Lecanicillium sp.The results of the indoor bioassay test showed that all of the Beauveria species were highly pathogenic to both larvae and adults of H.xiaoi,among which the ZR100 strain had the highest infection speed and the highest infection rate,and thus was considered as the most promising strain for future development and application.The results of the study provided important material basis and scientific grounds for biocontrol of Hylobitelus xiaoi Zhang.

关 键 词：萧氏松茎象 病原真菌 拟球孢白僵菌 球孢白僵菌 绿僵菌 轮枝菌 

分 类 号：S763.38[农业科学—森林保护学]