铁皮石斛种子组培繁育技术  

Tissue Culture Breeding Technology of Dendrobium officinale Seeds

作　　者：董薇[1] 余永亮[1] 杨红旗[1] 许兰杰[1] 谭政委 杨青 李磊 安素妨 鲁丹丹 梁慧珍[1] DONG Wei;YU Yong-liang;YANG Hong-qi(Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou,Henan 450002)

机构地区：[1]河南省农业科学院芝麻研究中心,河南郑州450002

出　　处：《安徽农业科学》2023年第21期177-179,共3页Journal of Anhui Agricultural Sciences

基　　金：国家现代农业产业技术体系(CARS-21);河南省农业科学院创新创意项目(2020CX03,2020CX19);河南省农业科学院优秀青年基金项目(2020YQ05,2020YQ28);河南省农业科学院自主创新专项基金项目(2021ZC72);河南省农业科学院新兴学科发展专项(2020XK07,2021XK03);河南省科技攻关项目(212102110297);河南省中央引导地方科技发展专项自由探索类项目(YDZX20214100001804)。

摘　　要：以铁皮石斛种子为材料,以马铃薯泥为培养基添加物,建立铁皮石斛种子实生苗组培体系。结果表明,种子萌发形成原球茎最佳培养基为1/2MS+10 g/L马铃薯泥+0.2 mg/L NAA,原球茎增殖分化培养基最佳培养基为MS+2.0 mg/L 6-BA+0.5 mg/L NAA+60 g/L马铃薯泥,壮苗生根最佳培养基为1/2MS+1.0 g/L活性炭+0.5 mg/L NAA+40 g/L马铃薯泥。Using Dendrobium officinale seeds as the material and potato mash as the medium additive,the tissue culture system of Dendrobium officinale seed was established.The results showed that the best medium for seed germination and formation of procorm was 1/2MS+10 g/L mashed potatoes+0.2 mg/L NAA;the best medium for procorm proliferation and differentiation was MS+2.0 mg/L 6-BA+0.5 mg/L NAA+60 g/L mashed potatoes;the best medium for rooting seedlings was 1/2MS+1.0 g/L activated carbon+0.5 mg/L NAA+40 g/L mashed potatoes.

关 键 词：铁皮石斛 种子 组培快繁 

分 类 号：S567.2[农业科学—中草药栽培]