HPLC测定驱虫药中二氯苯醚菊酯和吡虫啉的含量  

Determination of Permethrin and Imidacloprid in Anthelmintic Drops by HPLC

作　　者：刘冠麟 姜淋洁 王建 杨孝君 马吉飞 LIU Guan-lin;JIANG Lin-jie;WANG Jian(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300380;Tianjin Speerise Challenge Biotechnology Corporation,Tianjin 300080)

机构地区：[1]天津农学院动物科学与动物医学学院,天津300380 [2]天津市中升挑战生物科技有限公司,天津300080

出　　处：《安徽农业科学》2023年第21期180-183,193,共5页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立HPLC同时测定二氯苯醚菊酯吡虫啉滴剂中二氯苯醚菊酯、吡虫啉2种药效成分含量的方法。[方法]采用反向液相色谱法,色谱柱为X-Terra C_(18),流动相为0.05 mol/L甲酸铵溶液(pH=4.5)-乙腈,柱温35℃,梯度洗脱,检测波长235 nm。[结果]二氯苯醚菊酯、吡虫啉浓度分别在1.209 8~2.822 8、0.237 5~0.554 2 mg/mL时与峰面积呈现线性关系(R~2≥0.999 8);平均加样回收率分别为99.84%、100.33%,RSD均为0.51%;耐用性各项试验含量检测RSD均小于2%;精密度较高,样品溶液在24 h内稳定性良好。[结论]此方法可以准确便捷地测定二氯苯醚菊酯吡虫啉滴剂中药效成分的含量,适于药品的质量控制。[Objective]To establish a HPLC method for simultaneous determination of two active components(permethrin,imidacloprid)in dichlorobenzene permethrin imidacloprid drops.[Method]Reverse liquid chromatography was performed on X-Terra C 18 column with mobile phase consisted of 0.05 mol/L ammonium formate solution(pH=4.5)-acetonitrile,column temperature was 35℃,gradient elution was performed,and detection wavelength was 235 nm.[Result]There was a linear relationship between the peak area and the concentration of permethrin and imidacloprid at 1.2098-2.8228 and 0.2375-0.5542 mg/mL,respectively(R 2≥0.9998);the average sample recovery rates were 99.84%and 100.33%,respectively,the RSD was 0.51%.The durability tests RSD were all less than 2%.The sample solution had high precision and good stability within 24 hours.[Conclusion]This method can accurately and conveniently determine the content of active components in imidacloprid drops of dichlorobenzene permethrin,which is suitable for the quality control of drugs.

关 键 词：驱虫药 HPLC 方法学试验 二氯苯醚菊酯 吡虫啉 含量测定 

分 类 号：R927[医药卫生—药学]