UPLC-Q-TOF-MS/MS法分析栾樨叶的化学成分  被引量:6

Analysis of Chemical Constituents in the Leaves of Pluchea Indica(L.)Less.by UPLC-Q-TOF-MS/MS

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作  者:肖观林 江洁怡 程青云 张靖年 汤瑞茵 李冬梅[3] 李养学 XIAO Guan-lin;JIANG Jie-yi;CHENG Qing-yun;ZHANG Jing-nian;TANG Rui-yin;LI Dong-mei;LI Yang-xue(Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine,Guangdong Province Engineering Technology Research Institute of T.C.M.,Guangzhou 510095,China;Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan 528401,China;School of the Fifth Clinical Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区:[1]广东省中医药工程技术研究院广东省中医药研究开发重点实验室,广东广州510095 [2]广州中医药大学附属中山中医院/中山市中医院,广东中山528401 [3]广州中医药大学第五临床医学院,广东广州510405

出  处:《分析测试学报》2023年第11期1424-1433,共10页Journal of Instrumental Analysis

基  金:中山市社会公益与基础研究项目(210322223940650)。

摘  要:采用超高效液相色谱-四极杆-飞行时间高分辨质谱(UPLC-Q-TOF-MS/MS)对栾樨叶的化学成分进行定性分析。栾樨叶提取液经Waters UPLC BEH C_(18)柱(2.1 mm×100 mm,1.7μm)分离,以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,正、负离子模式下采集得到基峰色谱图。通过质谱数据库、化合物质谱裂解规律,并结合相关文献及对照品的保留时间与质谱信息,从栾樨叶中共鉴别出52个化学成分,包括2个香豆素类,15个黄酮类,16个苯丙素类,5个生物碱类,5个脂肪酸类,3个有机酸类,3个氨基酸类,1个酚酸类和2个其他类化合物,其中23个成分为栾樨叶中首次报道,11个化合物经对照品验证。该方法准确、可靠、高效,适用于栾樨叶化学成分的快速鉴定,可为栾樨叶的药效机制研究及临床应用提供依据。The main chemical constituents in the leaves of Pluchea indica(L.)Less.were qualitatively analyzed with ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS).The analytes were separated on a Waters UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)by gradient elution,with acetonitrile-0.1%formic acid solution as the mobile phase,and then analyzed in both positive and negative ion modes.A total of 52 chemical constituents were identified by mass spectrometry database,fragmentation regularities of mass spectra,consulting literature reports,retention time and mass data of reference compounds,including 2 coumarins,15 flavonoids,16 phenylpropanoids,5 alkaloids,5 fatty acids,3 organic acids,3 amino acids,1 phenolic acid,and 2 other compounds,of which 23 compounds were firstly reported for the leaves of Pluchea indica(L.)Less.,and 11 compounds were verified by reference substance.The method is accurate,reliable and efficient,which is suitable for rapid identification of ingredients in the leaves of Pluchea indica(L.)Less.,and can provide a basis for the pharmacodynamic mechanism and clinical application of the leaves of Pluchea indica(L.)Less.

关 键 词:阔苞菊  超高效液相色谱-四极杆-飞行时间高分辨质谱 化学成分 裂解规律 

分 类 号:O657.63[理学—分析化学] R284.1[理学—化学]

 

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