超高效液相色谱-串联质谱法测定化妆品中52种抗感染类药物  

Determination of 52 Anti-infective Drugs in Cosmetics by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：张丽蓉 肖紫芬 谢宋阳 毛林芳 王慧[2] 陈硕 ZHANG Li-rong;XIAO Zi-fen;XIE Song-yang;MAO Lin-fang;WANG Hui;CHEN Shuo(Fujian Institute for Food and Drug Quality Inspection,Fuzhou 350001,China;National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]福建省食品药品质量检验研究院,福建福州350001 [2]中国食品药品检定研究院,北京100050

出　　处：《分析测试学报》2023年第11期1503-1509,共7页Journal of Instrumental Analysis

摘　　要：建立了超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定化妆品中52种抗感染类药物的分析方法。样品经乙腈超声提取,选用Waters HSS T3色谱柱(2.1 mm×100 mm,1.8μm),以0.1%甲酸水-0.1%甲酸乙腈为流动相,在电喷雾(ESI)正、负离子模式下以多反应监测(MRM)模式进行测定。结果显示,水剂、乳液、膏霜3个基质中52种抗感染类药物在1~50 ng/mL范围内呈良好线性关系,相关系数(r)均大于0.99,检出限为1~10μg/kg,定量下限为3~35μg/kg。样品在3个加标水平下的回收率为81.2%~116%,相对标准偏差(RSD)均小于10%。该方法适用于化妆品中52种抗感染类药物的测定,可为化妆品中抗感染类药物的违禁添加监管提供技术参考。An ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was established for the simultaneous determination of 52 anti-infective drugs in cosmetics.After ultrasonic extraction with acetonitrile,the sample was eluted on Waters HSS T3 column(2.1 mm×100 mm,1.8μm)with mobile phase consisted of 0.1%formic acid solution-0.1%formic acid acetonitrile.For quantitative analysis,the electrospray ionization source was applied to carry out the positive and negative ions scanning with multiple reaction monitoring(MRM)mode.Results showed that there were good linear relationships for 52 anti-infective drugs from 1 ng/mL to 50 ng/mL,with correlation coefficients(r)greater than 0.99.The limits of detection ranged from 1 to 10μg/kg,and the limits of quantitation ranged from 3 to 35μg/kg.The recoveries ranged from 81.2%to 116%at three spiked levels,with the relative standard deviations(RSDs)less than 10%.The method could be used for the determination of 52 anti-infective drugs in cosmetics,and provides a technical reference for the supervision and administration of prohibited addition of anti-infective drugs in cosmetics.

关 键 词：超高效液相色谱-串联质谱法 化妆品 抗感染类药物 违禁添加 

分 类 号：O657.7[理学—分析化学] TQ658[理学—化学]