A"messenger zone hypothesis"based on the visual three-dimensional spatial distribution of motoneurons innervating deep limb muscles  

作　　者：Chen Huang Shen Wang Jin Deng Xinyi Gu Shuhang Guo Xiaofeng Yin 

机构地区：[1]MoE Key Laboratory for Trauma Treatment and Nerve Regeneration,Peking University,Beijing,China [2]Department of Orthopedics and Trauma,Peking University People’s Hospital,Beijing,China

出　　处：《Neural Regeneration Research》2024年第7期1559-1567,共9页中国神经再生研究（英文版）

基　　金：supported by the Chinese National General Program of the National Natural Science Foundation of China,No.82072162(to XY)。

摘　　要：Coordinated contraction of skeletal muscles relies on selective connections between the muscles and multiple classes of the spinal motoneuro ns.Howeve r,current research on the spatial location of the spinal motoneurons innervating differe nt muscles is limited.In this study,we investigated the spatial distribution and relative position of different motoneurons that control the deep muscles of the mouse hindlimbs,which were innervated by the obturator nerve,femoral nerve,inferior gluteal nerve,deep pe roneal nerve,and tibial nerve.Locations were visualized by combining a multiplex retrograde tracking technique compatible with three-dimensional imaging of solvent-cleared o rgans(3DISCO)and 3-D imaging technology based on lightsheet fluorescence microscopy(LSFM).Additionally,we propose the hypothesis that"messenger zones"exist as interlaced areas between the motoneuron pools that dominate the synergistic or antagonist muscle groups.We hypothesize that these interlaced neurons may participate in muscle coordination as messenger neurons.Analysis revealed the precise mutual positional relationships among the many motoneurons that innervate different deep muscles of the mouse.Not only do these findings update and supplement our knowledge regarding the overall spatial layout of spinal motoneurons that control mouse limb muscles,but they also provide insights into the mechanisms through which muscle activity is coordinated and the architecture of motor circuits.

关 键 词：3-D imaging MOTONEURONS multiple retrograde tracing muscle coordination skeletal muscle spatial distribution optical tissue clearing 

分 类 号：R338[医药卫生—人体生理学]