致倦库蚊取食前后肠道基因转录组学研究  

TRANSCRIPTOMIC ANALYSIS OF MIDGUT GENE OF CULEX QUINQUEFASCIATUS PRE-AND POST-FEEDING

作　　者：肖菲菲 黄旭[1,3] 肖颖 蔡怡珊[1] 黄恩炯 XIAO Fei-fei;HUANG Xu;XIAO Ying;CAI Yi-shan;HUANG En-jiong(Fuzhou International Travel Healthcare Center,Fuzhou 350001,Fujian,China;Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Fujian Medical University,Fuzhou 350122,Fujian,China;Technology Center of Fuzhou Customs,Fuzhou 350001,Fujian,China)

机构地区：[1]福州国际旅行卫生保健中心,福州350001 [2]福建农林大学,福州350002 [3]福建医科大学,福州350122 [4]福州海关技术中心,福州350001

出　　处：《寄生虫与医学昆虫学报》2023年第3期148-159,共12页Acta Parasitologica et Medica Entomologica Sinica

基　　金：福建省对外合作项目(2018I0023)。

摘　　要：为研究致倦库蚊取食前后肠道基因转录组水平的变化,对未取食、取食糖水、吸血条件下的致倦库蚊雌蚊肠道样品进行转录组高通量测序、分析筛选表达差异基因,并采用实时荧光定量PCR方法对结果进行验证。结果显示,在未取食和取食糖水条件下,共筛选出785个差异基因;在取食糖水和吸血条件下,共筛选出840个差异基因。转录组学分析发现,差异基因主要参与消化代谢、生长繁殖、免疫等过程。雌蚊羽化后与消化代谢、生殖相关的基因即开始表达,取食糖水和吸血后肠道中大部分基因表达量高于未取食状态。实时荧光定量PCR验证得到部分基因调控趋势与转录组测序基本一致。研究还发现细胞色素P450氧化酶、谷胱甘肽-S-转移酶等与杀虫剂代谢相关的解毒酶基因以及清道夫受体、丝氨酸蛋白酶抑制剂等与蚊虫自身免疫相关基因。本研究将有助于今后高效解毒酶类抑制剂杀虫剂的研发。Based on the High-throughput sequencing,a total of 13236013,11448509 and 13418598 reads were obtained from the non-feeding,sugar-feeding and blood-feeding Culex quinquefasciatus midguts,respectively,where 785 DEGs(differently expressed genes,DEGs)were screened out between unfed and sugar-feeding females of Cx quinquefasciatus midguts,and 840 DEGs were found between sugar-feeding and blood-feeding females.Functional annotation analysis(GO,KEGG,etc.)also suggests that the DEGs were mainly involved in digestion,metabolism,reproduction and immunization processes.Genes related to digestion,metabolism and reproduction of Cx.quinquefasciatus enriched after eclosion and the transcriptional enrichment soar significantly in sugar-feeding and blood-feeding females.qRT-PCR verifies the regulatory tendency described above,which was consistent with transcriptomic results.Cytochrome P450 oxygenase and glutathione S-transferase genes,which related to detoxifying enzymes and immune genes encoding scavengers and serine protease inhibitors in Cx.quinquefasciatus were found in present study,which would benefits the ongoing development of high-efficiency insecticides.

关 键 词：致倦库蚊 转录组测序 QRT-PCR 

分 类 号：R384.1[医药卫生—医学寄生虫学]