小亚璃眼蜱人工饲养条件下吸血期组织器官形态变化观察  

MORPHOLOGY OF TISSUES AND ORGANS OF HYALOMMA ANATOLICUM IN BLOOD-FEEDING UNDER ARTIFICIALLY REARING

作　　者：余权威 姚鹤松 妥小丽 梁欣悦 玉山·艾合麦特 何文文 伍军 诺明达来 罗亭祥 杨德鹏 胡政香 呼尔查[1,3] YU Quan-wei;YAO He-song;TUO Xiao-li;LIANG Xin-yue;YUSHAN Aihemai-te;HE Wen-wen;WU Jun;NUO Mingda-lai;LUO Ting-xiang;YANG De-peng;HU Zheng-xiang;HU Er-cha(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;College of Grass Land Science,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;Veterinary Medicine Post-doctoral Station of Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052 [2]新疆农业大学草业学院,乌鲁木齐830052 [3]新疆农业大学兽医学博士后流动站,乌鲁木齐830052

出　　处：《寄生虫与医学昆虫学报》2023年第3期160-165,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：新疆维吾尔自治区自然科学基金面上项目(2022D01A65);南疆地方肉羊疫病诊断及防治综合技术研究与示范项目(2021YFD1600702-3);新疆维吾尔自治区人力资源和社会保障厅人才项目“天池博士计划”;新疆农业大学动物医学学院大学生创新项目(dxscx2022194)。

摘　　要：小亚璃眼蜱Hyalomma anatolicum属于硬蜱科璃眼蜱属,是一种寄生于动物体表的专性吸血寄生虫,其分布范围广、寄生强度大。该蜱种可传播多种人畜共患病原体,对畜牧业造成巨大损失。为研究媒介蜱生物学特性和吸血期蜱类生理学变化,本研究利用实验室F2代小亚璃眼蜱进行人工饲养和组织器官切片染色,观察小亚璃眼蜱吸血期组织器官的形态学特征。结果显示,小亚璃眼蜱在实验条件下,从虫卵孵化为幼虫,再通过吸血成为若虫,进而吸血蜕皮为成蜱,共需要50.0 d。吸血期小亚璃眼蜱雌蜱苏木精伊红染色(HE染色)组织切片显示,随着雌蜱从半饱血至饱血状态,唾液腺细胞逐渐溶解消失,中肠腔内消化产生的血红素转移至中肠基底层和血淋巴中,然而卵巢组织并未出现明显变化。本研究首次观察了吸血期小亚璃眼蜱组织器官的变化,丰富了该蜱的生物学特性和组织学形态资料。Hyalomma anatolicum belongs to Ixodidae ticks,which is a species of obligate blood-feeding ectoparasites on animals and humans.It has a wide range of distribution,strong parasitic intensity,and spread a variety of zoonotic diseases and cause huge losses to animal husbandry annually.In order to study the physiological changes of ticks during blood-sucking period,the F2 generation of H.anatolicum were dissectioned and stained with Hematoxylin eosin(HE)stains to observe the morphological characteristics of tissues and organs during blood-sucking period.The results showed that under experimental conditions,it took 59.0 days for H.anatolicum to develop from eggs,hatch to larvae,feed and molt to nymphs,and then refeed and molt to adults to finish its whole life time.The tissue sections staining showed that as the female ticks became semi-engorged to engorged,the salivary gland cells gradually dissolved and disappeared.The heme produced by digestion in the midgut was transferred to the midgut basal layer and hemolymph,but there was no significant change in ovarian tissue.Our studies further explained the biological characteristics and histological morphology of H.anatolicum.

关 键 词：小亚璃眼蜱 人工饲养 器官形态 组织切片 

分 类 号：R384.4[医药卫生—医学寄生虫学]