四味生精口服液治疗肾阳虚模型大鼠实验研究  

Experimental study on the treatment of rat model with deficiency of kidney yang by Siwei Shengjing oral liquid

作　　者：郭丁禹 肖井雷[1] GUO Dingyu;XIAO Jinglei(Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学,长春130117

出　　处：《长春中医药大学学报》2023年第11期1211-1215,共5页Journal of Changchun University of Chinese Medicine

基　　金：2022年名贵中药资源可持续利用能力建设项目(2060302)。

摘　　要：目的探讨四味生精口服液对肾阳虚模型大鼠的治疗效果。方法通过氢化可的松肌肉注射(25 mg·kg^(-1)·d-1)建立大鼠肾阳虚模型,连续造模14 d。造模后分为空白对照组、模型对照组、阳性对照组(金匮肾气丸每公斤4.5粒)、四味生精口服液高(3.6 mL·kg^(-1))、中(1.8 mL·kg^(-1))、低剂量组(0.9 mL·kg^(-1)),共6组。第15天起,按10 mL·kg^(-1)灌胃给药,空白对照组、模型对照组灌胃给予等体积蒸馏水,每天1次,连续给药14 d。末次给药后,取睾丸、前列腺、附睾,计算脏器指数;计算精子总数并检测精子存活率;检测血清睾酮(T)与雌二醇(E2)水平;观察睾丸形态变化,测量生精小管上皮细胞层高度(μm)和生精小管横截面中生精细胞所占面积(μm2)。结果与模型对照组比较,性激素水平:各组T含量均明显升高(P<0.001);所有组别E2含量均明显降低(P<0.001)。脏器系数:各组睾丸、附睾、前列腺脏器系数均无明显差异(P>0.05)。精子数与存活率:各组均精子总数均明显升高(P<0.01,P<0.001);各组精子存活率均有明显升高(P<0.01,P<0.001)。病理检测:各给药组具有改善和促进睾丸生精的作用。且各给药组均可以明显增加模型大鼠睾丸生精小管上皮厚度与生精细胞横截面积(P<0.05,P<0.01,P<0.001)。结论四味生精口服液对肾阳虚模型大鼠具有较好的治疗作用。Objective To explore the therapeutic effect of Siwei Shengjing oral liquid on rat model with deficiency of kidney yang.Methods Rat model with deficiency of kidney yang was established by intramuscular injection of hydrocortisone(25mg·kg^(-1)·d-1)for continuous 14 days.After modeling,they were divided into six groups,namely the blank control group,the model control group,the positive control group(Jingui Shenqi Pill 4.5 capsules/kg),and high dose(3.6 mL·kg^(-1))group,medium dose(1.8mL·kg^(-1))group and low dose(0.9 mL·kg^(-1))group of Siwei Shengjing oral liquid.From the 15th day,the drug was administered by gavage at the dose of 10 mL·kg^(-1),while the blank control group and the model control group were given equal volumes of distilled water by gavage once a day for 14 consecutive days.After the last administration,the testis,prostate and epididymis were taken,and the organ indexes of these organs were calculated separately.The total number of sperm was calculated,and the sperm survival rate was measured.The serum testosterone(T)and estradiol(E2)levels were detected.The morphological changes of the testis were observed,and the height of the epithelial cell layer(μm)and the area of the seminiferous cells in the cross-section(μm2)of the seminiferous tubules were measured.Results The results compared with the model control group were as follows.The levels of sex hormones showed that the T content in each group significantly increased(P<0.001),and the E2 content in all groups significantly decreased(P<0.001).There were no statistically significant differences in the organ coefficients of testis,epididymis and prostate in each group(P>0.05).The total number of sperm in each group was significantly increased(P<0.01,P<0.001);The sperm survival rate in each group was significantly increased(P<0.01,P<0.001).In terms of pathological examination,each administered group had the effect of improving and promoting testicular spermatogenesis,and each administered group could significantly increase the thickness of the t

关 键 词：四味生精口服液 肾阳虚 性激素 精子数 精子存活率 病理检测 

分 类 号：R285[医药卫生—中药学] R289[医药卫生—中医学]