复合菌群强化修复石油-氯酚污染土壤  

Enhanced Remediation of Petroleum-Chlorophenol Contaminated Soil by Compound Bacteria

作　　者：李婧[1] 于钧泷 赵晓东[1] 左帅 刘其友[1] 张秀霞[1,2] LI Jing;YU Junlong;ZHAO Xiaodong;ZUO Shuai;LIU Qiyou;ZHANG Xiuxia(College of Chemistry and Chemical Engineering,China University of Petroleum,Qingdao 266580,China;State Key Laboratory of Pollutants Control and Pretreatment in Petroleum and Petrochemical Industry,Beijing 102206,China)

机构地区：[1]中国石油大学(华东)化学化工学院,山东青岛266580 [2]石油石化污染物控制与处理国家重点实验室,北京102206

出　　处：《石油学报（石油加工）》2023年第6期1412-1422,共11页Acta Petrolei Sinica(Petroleum Processing Section)

基　　金：中央高校基本科研业务费专项资金(22CX01004A-6);石油石化污染物控制与处理国家重点实验室开放基金(PPC2019021,PPC2020015);中国石油科技创新基金研究项目(2018D-5007-0605)资助。

摘　　要：为了解决石油烃和有机氯化合物复合污染问题,采用石油-有机氯复合菌群,以2,4-二氯苯酚为代表性有机氯化合物,开展石油-氯酚复合污染土壤生物修复实验,考察修复过程中污染物降解率、微生物总数、酶活性及群落结构变化。结果表明:添加石油-有机氯复合菌群修复污染土壤130 d后,石油烃降解率达到77.68%,碳数27以下所有烃类峰强度均有降低,氯酚降解率达到100%。修复过程中土壤脱氢酶活性、细菌和真菌数量呈先上升后下降趋势,且三者与石油烃和氯酚降解率呈正相关,修复后土壤微生物Alpha多样性指数下降。利用高通量测序技术检测微生物群落结构。结果表明:菌群强化修复的土壤中厚壁菌门比例增加,放线菌门比例降低;添加复合菌群强化修复后土壤中芽孢杆菌属和苍白杆菌属为优势菌属。To solve the problem of co-contamination of petroleum hydrocarbons and organochlorine compounds,the bioremediation experiment of petroleum-chlorophenol co-contaminated soil was carried out using petroleum-organochlorine compound bacteria as the material and 2,4-dichlorophenol as the representative organochlorine compound,so as to investigate the pollutant degradation rate,total number of microorganisms,enzymatic activity and changes in community structure.The results show that 130 days after remediation of the contaminated soil with petroleum-organochlorine compound bacteria,the degradation rate of petroleum hydrocarbons reached 77.68%,the peak intensity of all hydrocarbons with the carbon number below 27 decreased,and the degradation rate of chlorophenol reached 100%.During the remediation process,the activity of soil dehydrogenase,the number of bacteria and fungi increased first and then decreased,and these three factors were positively correlated with the degradation rates of petroleum hydrocarbons and chlorophenols.After remediation,Alpha diversity index of soil microorganisms decreased.High-throughput sequencing technology was used to detect the microbial community structure.The results show that the proportion of Firmicutes increased and that of Actinobacteria decreased in the soil with enhanced microbial restoration.Bacillus and Ochrobactrum became dominant bacteria in the soil after adding the compound bacteria to strengthen the remediation.

关 键 词：复合菌群 石油 氯酚 污染土壤 生物修复 群落结构 降解率 

分 类 号：X53[环境科学与工程—环境工程]