北京市急性腹泻患儿中A组双埃可病毒的感染状况及基因型研究  

Epidemiological characteristics and genotypes of parechovirus A from children with acute diarrhea in Beijing

作　　者：吉彦莉[1] 王永全[1] 崔海洋[1] 靳博[1] 刘潇潇[1] 黄艳红 JI Yanli;WANG Yongquan;CUI Haiyang;JIN Bo;LIU Xiaoxiao;HUANG Yanhong(Xicheng Center for Disease Control and Prevention,Beijing 100120,China)

机构地区：[1]北京市西城区疾病预防控制中心,北京100120

出　　处：《公共卫生与预防医学》2023年第6期21-25,共5页Journal of Public Health and Preventive Medicine

基　　金：首都卫生发展科研专项(首发2020-3-7022)。

摘　　要：目的对2021年北京市急性腹泻患儿A组双埃可病毒(parechovirus A,PeV-A)的感染状况及基因型别进行研究。方法2021年1~12月,每月随机抽取北京市腹泻监测某哨点医院门诊就诊的<60月龄急性腹泻患儿粪便标本。提取病毒核酸,通过实时荧光RT-PCR检测PeV-A。用巢式RT-PCR扩增PeV-A的VP3/VP1连接区,通过测序和NCBI BLAST程序比对确定PeV-A基因型别;用实时荧光PCR对A组轮状病毒、诺如病毒、肠道腺病毒、星状病毒和札如病毒进行检测,分析混合感染状况。利用生物信息学及统计软件分别完成进化和统计分析。结果198例粪便标本中,PeV-A阳性11例,检出率为5.56%(11/198)。其中,2例混合肠道腺病毒感染。81.82%(9/11)的PeV-A阳性患儿<24月龄。秋季检出率最高,为12.50%(7/56)。90.91%(10/11)的PeV-A感染集中在夏秋两季。11例阳性标本中有9例测序成功,其中,7例为PeV-A1B型,2例为PeV-A3型。结论2021年北京市急性腹泻患儿感染PeV-A共检出PeV-A1B和PeV-A3两种型别,2岁以内婴幼儿是PeV-A感染的好发人群。腹泻患儿感染PeV-A主要集中在夏秋两季。Objective To investigate the epidemiological chatacteristics and genotypes of parechovirus A(PeV-A)from children with acute diarrhea in Beijing in 2021.Methods Fecal samples were randomly collected from outpatient children under 60 months with acute diarrhea in a sentinel hospital in Beijing from January to December of 2021.RNA was extracted and detected for PeV-A by real-time RT-PCR.Nested RT-PCR was performed to amplify the VP3/VP1 conjunction region.PeV-A genotypes were determined based on sequencing and NCBI BLAST.Group A rotavirus,norovirus,enteric adenovirus,astrovirus and sapovirus were also detected for co-infection analysis.Phylogenetic and statistical analyses were performed using bioinformatics and statistical software.Results Of the 198 stool samples,11 were positive for PeV-A,with a detection rate of 5.56%(11/198).Among them,2 cases were co-infected with enteric adenovirus.81.82%(9/11)of PeV-A infected cases were under 24 months.The highest detection rate was observed in fall,which was 12.50%(7/56).90.91%(10/11)of PeV-A infection occurred in summer and fall.Among the 11 PeV-A isolates,9 were sequenced successfully,of which 7 belonged to PeV-A1B genotype and 2 belonged to PeV-A3 genotype.Conclusion PeV-A1B and PeV-A3 are identified in children with acute gastroenteritis in Beijing in 2021.Infants and young children under 2 years old are the high-risk population for PeV-A infection.Most infections occur in summer and fall.

关 键 词：A组双埃可病毒 急性腹泻 基因型 

分 类 号：R181[医药卫生—流行病学]