泽陆蛙变态前后身体和器官大小及PHA-P反应的变化  

Variations of body and internal organ size,and responsiveness to phytohemagglutinin before and after metamorphosis in Fejervarya multistriata

作　　者：伍亮 王月妍 李文慧 刘志冲 梁艺博 杨华珍 许梅 赵艺洋 张志强[1] WU Liang;WANG Yueyan;LI Wenhui;LIU Zhichong;LIANG Yibo;YANG Huazhen;XU Mei;ZHAO Yiyang;ZHANG Zhiqiang(School of Animal Science and Technology,Anhui Agricultural University,Hefei 230036)

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2023年第5期817-822,共6页Journal of Anhui Agricultural University

基　　金：安徽农业大学2021年度省级线下课程项目(2021xxkc039);校级质量工程培育项目一流教材项目(2021auyljc01);安徽农业大学2021年校级创新训练项目(XJDC202110364044);2022年国家级(202210364096X)大学生创新创业训练项目共同资助。

摘　　要：为探究蝌蚪变态前后形态表型、内脏器官大小和免疫功能与特定生活史阶段的关系,测定了泽陆蛙(Fejervarya multistriata)从37期蝌蚪发育至46期幼蛙,多个关键生活史阶段身体大小、内脏器官系数及对植物血凝素(phytohemagglutinin,PHA-P)的反应的变化。结果显示:(1)泽陆蛙的体重、体宽、尾长和体全长都从37期至40~41期升高,之后下降,至46期降至最低值(P<0.05);体长42~43期最高,显著高于37期和46期(P<0.05)。(2)肝、脂肪体和肾湿重系数都在44~45期最高,38~39期最低(P<0.05),而鳃或肺湿重系数37期显著高于其他发育阶段(P<0.05),消化道长度系数37期、38~39期和40~41期都显著高于42~43期、44~45期和46期(P<0.05),心和脾湿重系数均不随发育阶段而变化(P>0.05)。(3)对PHA-P的反应的最大反应值37期蝌蚪出现于注射后2 h,38~39和42~43期见于3 h,其他发育阶段见于4 h,42~43期蝌蚪显著高于38~39期蝌蚪和46期幼蛙(P<0.05)。从水生到陆生,泽陆蛙的形态表型变化先于内脏器官大小,以被延迟和下降的免疫功能为代价,影响其成功登陆。To explore the relationship between morphological phenotypes,internal organ size and immune function and specific life history phases before and after metamorphosis in rice frogs,Fejervarya multistriata,tadpoles at different developmental stages from Gosner 37-stage to Gosner 46-stage were sampled,and the varia-tions of body size,internal organ index and the responsiveness to phytohemagglutinin(PHA-P)for multiple key life history phases were measured,respectively.The results were as follows:(1)The body mass,body width,tail length,and total body length was gradually increased from Gosner stage 37 to 40-41 tadpoles,and then declined with a minimum at Gosner stage 46 froglets(P<0.05).The snout to vent length showed the highest value at Gosner stage 42-43 tadpoles,which was significantly higher than that of Gosner stage 37 tadpoles and Gosner stage 46 froglets(P<0.05).(2)The wet mass index of livers,fat bodies and kidneys peaked at Gosner stage 44-45 tadpoles,all showed the minimum value at Gosner stage 38-39 tadpoles(P<0.05);The wet mass index of gill or lungs peaked at Gosner stage 37 tadpoles,which was significantly higher than that of any other developmental stage(P<0.05);The length index of digestive tract was greatly higher at Gosner stage 37,38-39 and 40-41 tad-poles than that of Gosner stage 42-43 and 44-45 tadpoles and Gosner stage 46 froglets(P<0.05).No significant developmental stage differences were found for the wet mass index of heart and spleen(P>0.05).(3)The maxi-mal value for the PHA-P response of Gosner stage 37,38-39 and 42-43 tadpoles appeared 2 h,3 h,and 4 h after injection,respectively,and 4 h after injection for any other developmental stage,which was significantly higher at Gosner stage 42-43 tadpoles than that of Gosner stage 38-39 tadpoles and Gosner stage 46 froglets(P<0.05).From aquatic to terrestrial environments,the variations of morphological phenotype occur prior to dramatic estab-lishments and reconstruction of internal organ sizes in Fejervarya multistriata,delayed and decreased immu

关 键 词：泽陆蛙 变态发育 形态表型 内脏器官大小 植物血凝素 

分 类 号：S917.4[农业科学—水产科学]