一株水华鱼腥藻溶藻细菌的分离鉴定和溶藻特性  

Isolation and characterization of algae-solubilizing bacteria from a waterwash strain of cichlidium ichneumoniae

作　　者：洪桂云 黄浚峰 朱钰妮 朱曙光 HONG Guiyun;HUANG Junfeng;ZHU Yu'ni;ZHU Shuguang(College of Environment and Energy Engineering,Anhui Jianzhu University,Engineering Research Center of Building Energy Effi-ciency Control and Evaluation,Ministry of Education,Anhui Province,Hefei 230601)

机构地区：[1]安徽建筑大学环境与能源工程学院,安徽省建筑能效控制与评估教育部工程研究中心,合肥230601

出　　处：《安徽农业大学学报》2023年第5期881-886,共6页Journal of Anhui Agricultural University

基　　金：安徽省自然科学基金(No.1908085141ME);校级教研项目(No.2021jy27)共同资助。

摘　　要：从不同来源的水体和土样中,筛选出对水华鱼腥藻具有溶藻作用的溶藻菌株SLW6。对菌株SLW6进行形态特征分析和生理生化鉴定,及16S rDNA序列分析,并研究了溶藻菌在不同培养时期、不同pH条件下对菌株SLW6溶藻效果的影响,探究了该菌株对水华鱼腥藻的溶藻方式。结果表明:菌株SLW6呈革兰氏阴性,V-P试验、明胶液化试验、甲基红试验为阴性,过氧化氢试验、硝酸盐还原试验都为阳性,菌株SLW6与产碱杆菌属的Alcaligenes faecalis(NR113606.1)的同源性达到99.65%,结合形态学特征,生理生化鉴定和16S rDNA序列分析,初步判断溶藻菌株SLW6为产碱菌属(GenBank登录号为OP363821)。超声辅助热乙醇提取叶绿素a,分光光度法测定叶绿素a含量,用叶绿素a含量变化计算溶藻率,结果表明SLW6处于对数期时的溶藻效果最好,溶藻率达89.45%;在pH=7条件下,溶藻效果较好,溶藻率为47.03%;溶藻菌SLW6对水华鱼腥藻的作用方式是直接溶藻为主间接溶藻作用为辅。The algolytic strain SLW6 was screened from water and soil samples from different sources,which has the effect of algicidal activity on Anabaena flos aquatica.The morphological characteristics,physiological and biochemical identification and 16S rDNA sequence analysis of strain SLW6 were carried out.The effects of algaeclytic bacteria on algaeclytic effect of strain SLW6 in different culture periods and pH conditions were stud-ied.The pattern of dissolving Anabaena flos aquae by the strain was explored.The results showed that:strain SLW6 was a Gram-negative bacterium,negative in V-P test,gelatin liquefaction test and methyl red test,while positive in hydrogen peroxide test and nitrate reduction test;the homology between SLW6 strain and Alcaligenes faecalis(NR113606.1)strain was 99.65%;combined with morphological characteristics,physiological and bio-chemical identification and 16S rDNA sequence analysis,it was preliminarily determined that the algae lytic strain SLW6 was an alkali producing bacterium(GenBank accession number,OP363821).Ultrasound assisted hot etha-nol extraction of the chlorophyll a,determination of chlorophyll a content by spectrophotometry,and calculation of the algal dissolution rate based on the change of chlorophyll a content,the results showed that SLW6 had the best algae dissolving effect when it was in the logarithmic phase,and the algae dissolving rate was 89.45%;when pH was 7,the effect of algae dissolution was better,and the algae dissolution rate was 47.03%;the mode of action of alginolytic bacteria SLW6 on Anabaena flos aquatica was that the direct alginolytic activity is dominant and the indirect alginolytic activity is secondary.

关 键 词：溶藻菌 水华鱼腥藻 分离鉴定 溶藻特性 溶藻方式 

分 类 号：X52[环境科学与工程—环境工程]