季也蒙毕赤酵母合成碳点检测四环素  

Carbon dots synthesized by Meyerozyma guilliermondii for tetracycline detection

作　　者：张亚维 任倩文 万光仪 武超[1,2] ZHANG Yawei;REN Qianwen;WAN Guangyi;WU Chao(School of Resources and Environmental Engineering,Anhui University,Hefei 230601;Anhui Province Key Laboratory of Industrial Wastewater and Environmental Treatment,Hefei 230601;Institutes of Physical Science and Information Technology,Anhui University,Hefei 230601)

机构地区：[1]安徽大学资源与环境工程学院,合肥230601 [2]工业废水及环境治理安徽省重点实验室,合肥230601 [3]安徽大学物质科学与信息技术研究院,合肥230601

出　　处：《安徽农业大学学报》2023年第5期897-903,共7页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(32170135);工业废水及环境治理安徽省重点实验室项目(DHSZ202204)共同资助。

摘　　要：为了探究碳点(CDs)在生物传感方面的应用,以季也蒙毕赤酵母菌(Meyerozyma guilliermondii PG-1)为原料,通过一步水热合成法成功制备了水溶性良好、光学性质稳定的蓝色荧光碳点,并通过透射电子显微镜、傅里叶红外光谱、X射线电子衍射和荧光光谱等方法对CDs进行表征。获得的CDs近似圆形,尺寸在11 nm左右,表面含有大量亲水性官能团,432 nm处有最强的发射光谱。进一步将CDs用于水中微量盐酸四环素(TC)的检测。结果显示,基于静态猝灭和内滤效应,TC能够快速猝灭碳点荧光,荧光猝灭效率在0.02~0.18 g·L^(-1)浓度范围内有良好的线性关系,表明碳点作为荧光探针检测TC的潜在应用前景。In order to explore the application of carbon dots(CDs)in biosensors,this paper successfully pre-pared blue fluorescent carbon dots with excellent water solubility and stable optical properties by one-step hydro-thermal synthesis using Meyerozyma guilliermondii PG-1 as raw material,and characterized CDs by transmission electron microscopy,Fourier infrared spectroscopy,X-ray electron diffraction and fluorescence spectroscopy.The obtained CDs were approximately round,with a size of about 11 nm,a large number of hydrophilic functional groups on the surface,and the strongest emission spectrum at 432 nm.CDs was further applied to the determina-tion of trace tetracycline hydrochloride(TC)in water.The results showed that TC can rapidly quench the fluores-cence of carbon dots based on static quenching and internal filtering effect,and the fluorescence quenching effi-ciency has a good linear relationship in the concentration range of 0.02-0.18 g·L^(-1),indicating its potential applica-tion as a fluorescent probe for TC detection.

关 键 词：碳点 季也蒙毕赤酵母菌 荧光探针 盐酸四环素 荧光猝灭 

分 类 号：X835[环境科学与工程—环境工程] O657.3[理学—分析化学]