四逆汤对溃疡性结肠炎大鼠TLR4/NF-κB信号通路相关因子及肠道菌群的影响  

Effects of Sini Decoction(四逆汤)on TLR4/NF-κB Signaling Pathway Related Factors and Intestinal Flora in Rats with Ulcerative Colitis

作　　者：陈泰宇[1] 唐学贵[1] 蒋小东 唐诗宇 孔鹏飞[1] 邓琴 彭钰蓉 谭雯心 CHEN Taiyu;TANG Xuegui;JIANG Xiaodong;TANG Shiyu;KONG Pengfei;DENG Qin;PENG Yurong;TAN Wenxin(Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院附属医院,四川南充637000

出　　处：《中华中医药学刊》2023年第10期83-87,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(82074429);南充市科技局市校科技战略合作专项基金(22SXQT0076)。

摘　　要：目的 探讨四逆汤对溃疡性结肠炎大鼠Toll样受体4(Toll-like receptor 4,TLR4)/核因子-κB(nuclear factor-κB,NF-κB)信号通路相关因子及肠道菌群的影响。方法 选取健康雄性SD大鼠28只,常规饲料喂养,自由进食进水,实验开始前适应性饲养7 d。按照随机数字表法将28只大鼠分为正常组、模型组、四逆汤组、美沙拉嗪组,各7只。正常组大鼠注入氯化钠溶液,模型组、四逆汤组、美沙拉嗪组大鼠通过结肠内注入三硝基苯磺酸(trinitrobenzenesulfonic acid, TNBS)建立溃疡性结肠炎模型。造模完成后,四逆汤组大鼠采用四逆汤3.8 g/kg灌胃,美沙拉嗪组采用美沙拉嗪10 g/kg灌胃,1次/d。在给药前1 d及给药7 d后秤测量大鼠的体质量、疾病指数活动评分(disease activity index, DAI)评分,检测大鼠给药7 d后的TLR4、NF-κB、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-10(interleukin-10,IL-10)、白细胞介素-16(interleukin-16,IL-16)、黏蛋白2(mucin 2,MUC2)、三叶因子3(trefoil factor 3,TFF3)表达及肠道菌群。结果 给药前,正常组大鼠体质量显著高于模型组、四逆汤组、美沙拉嗪组(P<0.05);给药7 d后,四逆汤组、美沙拉嗪组大鼠体质量显著升高(P<0.05),正常组、四逆汤组、美沙拉嗪组大鼠体质量显著高于模型组(P<0.05)。给药前,正常组大鼠DAI评分显著低于模型组、四逆汤组、美沙拉嗪组(P<0.05);给药7 d后,四逆汤组、美沙拉嗪组大鼠DAI评分显著降低(P<0.05),模型组、四逆汤组、美沙拉嗪组大鼠DAI评分显著高于正常组(P<0.05),四逆汤组、美沙拉嗪组大鼠DAI评分显著低于模型组(P<0.05)。给药7 d后,正常组、四逆汤组、美沙拉嗪组大鼠TLR4、NF-κB表达均显著低于模型组(P<0.05)。给药7 d后,正常组、四逆汤组、美沙拉嗪组大鼠TNF-α、IL-10、IL-16水平均显著低于模型组(P<0.05)。给药7 d后,正常组、四逆汤组、美沙拉嗪组大鼠肠球菌、肠杆菌显�Objective To investigate the effects of Sini Decoction(四逆汤)on Toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB)signaling pathway related factors and intestinal flora in rats with ulcerative colitis.s Methods Twenty-eight healthy male Sprague-Dawley(SD)rats were randomly fed with conventional diet and water intake.The rats were fed adaptively for 7 days before the experiment.According to the random number table method,28 rats were divided into normal group,model group,Sini Decoction group and mesalazine group,7 rats in each group.The rats in the normal group were injected with sodium chloride solution,and the rats in the model group,the Sini Decoction group and the mesalazine group were injected with trinitrobenzenesul-fonic acid(TNBS)into the colon to establish the ulcerative colitis model.After the modeling,the rats in Sini Decoction group were given Sini Decoction 3.8 g/kg by gavage,and the rats in mesalazine group were given mesalazine 10 g/kg by gavage,once a day.The body weight and disease activity index(DAI)of rats were measured 1 day before and 7 days after administration.The expressions of TLR4,NF-κB,tumor necrosis factor-α(TNF-α),interleukin-10(IL-10),interleukin-16(IL-16),mucin 2(MUC2)and trefoil factor 3(TFF3)and intestinal flora of rats were detected 7 days after administration.s Results Before admin-istration,the body weight of the rats in the normal group was significantly higher than that in the model group,the Sini Decoction group and the mesalazine group(P<0.05).After 7 days of administration,the body weights of rats in the Sini decoction group and the mesalazine group were significantly increased(P<0.05),and the body weights of the rats in the normal group,the Sini Decoction group and the mesalazine group were significantly higher than those in the model group(P<0.05).Before administra-tion,the DAI score of the normal group was significantly lower than that of the model group,the Sini Decoction group and the me-salazine group(P<0.05).After 7 days of administration,the DAI scores of

关 键 词：四逆汤 溃疡性结肠炎大鼠 TOLL样受体4 核因子-ΚB 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]