红蓝光质调控烟苗根系发育的机理研究  

Mechanism of Red or Blue Light in Regulating Root Development of Tobacco Seedlings

作　　者：林智慧[1] 王雪仁[1] 陈涛[1] 陈承亮 孟霖[2] 宋文静[2] LIN Zhihui;WANG Xueren;CHEN Tao;CHEN Chengliang;MENG Lin;SONG Wenjing(Sanming Tobacco Company of Fujian Province,Sanming,Fujian 365000,China;Institute of Tobacco Research,CAAS,Qingdao,Shandong 266101,China)

机构地区：[1]福建省烟草公司三明市公司,福建三明365000 [2]中国农业科学院烟草研究所,山东青岛266101

出　　处：《福建农业学报》2023年第8期910-916,共7页Fujian Journal of Agricultural Sciences

基　　金：中国烟草总公司福建省公司科技项目(2021350000240011);山东省自然科学基金项目(ZR2019BC066)。

摘　　要：【目的】探究红蓝光质调控烟苗根系发育的机理。【方法】以烤烟品种翠碧1号为试材,采用水培试验,研究红蓝光对烟草幼苗根系生长、内源气体信号分子硫化氢(H2S)合成和积累的影响。【结果】处理7 d时,与对照白光处理相比,结果表明:(1)红光处理烟苗地上部和根系生物量显著增加,增幅分别为74.62%和15.64%,蓝光根系生物量降幅为7.00%;(2)红光处理烟苗根系H2S含量显著增加,增幅为61.72%,而蓝光处理显著降低;(3)红光处理烟苗根系脱巯基酶(DES)、半胱氨酸合成酶(CS)活性分别升高24.28%、25.61%;蓝光处理则分别降低32.10%、18.30%;(4)红光处理的H2S生物合成关键基因NtDES表达量为白光处理的8.8倍,蓝光处理仅为白光处理的13.82%。【结论】红光处理可通过增强H2S生物合成关键酶编码基因的表达提高烟苗根系H2S的含量,进而促进侧根的发生,而蓝光效应与红光相反。【Objective】Mechanism of red or blue light in regulating the root development of flue-cured tobacco seedlings was studied.【Method】Effects of red or blue LED light exposure on the root growth and the synthesis and accumulation of endogenous gas signal molecule hydrogen sulfide(H2S)in CB-1 tobacco seedlings were monitored.【Results】After 7 days of the exposure to red or blue LED in comparison to white light,(1)the biomasses of aboveground parts and roots of the seedlings treated by red light increased by 74.62%and 15.64%,respectively,while the roots of the seedlings exposed to the blue LED decreased by 7.00%;(2)the H2S content on the red light-treated plants increased by 61.72%,but reduced on the blue light-treated counterparts;(3)the red LED increased the activities of DES and CS by 24.28%and 25.61%,respectively,but the blue LED decreased them by 32.10%and 18.30%,respectively;(4)the NtDES expression in the red light-treated seedlings were 8.8-fold of that in the plants treated by white light,whereas the blue light resulted in merely 13.82%of what white light did.【Conclusion】In contrast to blue LED,the exposure of the tobacco seedlings to red LED rose H2S in the plants through increased expression of the key enzyme coding genes in the biosynthesis inducing a thriving generation of lateral roots.

关 键 词：光质 烟苗 侧根 硫化氢 

分 类 号：S572[农业科学—烟草工业]