滇杨性别连锁的SSR标记  

SSR Markers Associated with Sex-difference of Populus yunnanensis Dode

作　　者：李启少 雷瀚 沈伟祥 赵文植 王飞[1,2] 徐剑 瞿绍宏 董章宏 辛培尧[1,2] LI Qishao;LEI Han;SHEN Weixiang;ZHAO Wenzhi;WANG Fei;XU Jian;QU Shaohong;DONG Zhanghong;XIN Peiyao(Southwest Research Center for Landscape Architecture Engineering of State Forestry and Grassland Administration,Southwest Forestry University,Kunming,Yunnan 650224,China;Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China,Southwest Forestry University,Kunming,Yunnan 650224,China;Beijing Science and Technology Development Center of Ministry of Agriculture and Rural Affairs,Beijing 102600,China;Kaiyuan Baituqiang National Forest Farm,Kaiyuan,Yunnan 661600,China)

机构地区：[1]西南林业大学国家林业和草原局西南风景园林工程技术研究中心,云南昆明650224 [2]西南林业大学西南地区生物多样性保育国家林业和草原局重点实验室,云南昆明650224 [3]北京市农业农村部科技发展中心,北京102600 [4]开远市白土墙国有林场,云南开远661600

出　　处：《福建农业学报》2023年第8期953-959,共7页Fujian Journal of Agricultural Sciences

基　　金：云南省科技人才与平台计划项目(202205AF150022)。

摘　　要：【目的】在滇杨幼苗时期和开花前利用形态差异对雌雄株进行性别鉴定极为困难,因此亟需探明对其性别进行快速鉴定的分子标记方法。【方法】以滇杨雌雄各30株为材料,利用简单重复序列(SSR)筛选与滇杨性别相关的分子标记,并利用所获得的SSR标记对未知性别滇杨幼苗进行鉴定,分析滇杨苗期的性别分化情况。【结果】从已报道的相关文献中选出15对与植物性别连锁的SSR引物,经试验筛选出1对(BPCA90)在琼脂糖凝胶检测中显现清晰条带且反应稳定的SSR引物,并将这对引物经聚丙烯酰胺凝胶电泳进行复筛,经PCR扩增后在滇杨雄株中出现1条特异性条带,大小在680 bp左右。利用这对SSR引物对30株滇杨幼苗进行性别鉴定,结果表明雄株明显多于雌株,且比例为13∶2。【结论】试验筛选所得到的SSR引物可以用于滇杨早期进行性别鉴定。研究结果可作为滇杨早期性别鉴定的遗传标记,为今后滇杨在生产中的分性别利用提供技术支持。【Objective】Means utilizing molecular markers to differentiate rapidly and accurately male and female Populus yunnanensis plants at young stages was investigated.【Method】Using simple sequence repeats(SSR),molecular markers were screened for those might associate with the sex of P.yunnanensis at seedling stages and before flowering.【Result】Fifteen pairs of SSR primers were selected from the reported literatures.One of them,coded BPCA90,showed clear bands and stable reaction in agarose gel detection.After rescreening the primer by polyacrylamide gel electrophoresis,a specific band of approximately 680 bp appeared in the PCR amplification of male P.yunnanensis.Subsequent testing on 30 plants using these SSR primers showed significantly more males than females in a ratio of 13 to 2.【Conclusion】Short of a visible morphological sign for the determination,the SSR primers screened by this study appeared to suffice the general purpose of differentiating the sex of P.yunnanensis at early growth stage.

关 键 词：滇杨 SSR 引物筛选 性别鉴定 

分 类 号：S792[农业科学—林木遗传育种]