猪圆环病毒2型复制酶蛋白的胞内互作蛋白研究进展  

Research Progress on Intracellular Proteins Interacting with PCV2 Rep

作　　者：陈羽彤 田淑婧 陆春秀 苏春宇 吕其壮 黄维 CHEN Yutong;TIAN Shujing;LU Chunxiu;SU Chunyu;LV Qizhuang;HUANG Wei(College of Biology&Pharmacy,Yulin Normal University,Yulin,Guangxi 537000,China;Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,Yulin,Guangxi 537000,China)

机构地区：[1]玉林师范学院生物与制药学院,广西玉林537000 [2]广西农产资源化学与生物技术重点实验室,广西玉林537000

出　　处：《福建农业学报》2023年第8期1004-1010,共7页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31860708);玉林师范学院大学生创新创业训练计划项目(202210606027)。

摘　　要：猪圆环病毒2型(Porcine circovirus virus type 2,PCV2)是引起断奶仔猪多系统衰竭综合征(Postweaning multisystemic wasting syndrome,PMWS)以及其他猪相关疾病的主要病原体。复制酶(Replicase,Rep)蛋白是由PCV2 ORF1基因编码的功能性复制启动蛋白,通过与ORF1编码的另一复制相关蛋白Rep′共同作用参与PCV2的DNA滚环复制。Rep蛋白作为PCV2的一种必需功能性蛋白,是研究PCV2抗病毒治疗的一个新突破口。然而,目前有关PCV2致病机理及疫苗的研究大多集中在Cap蛋白,对于Rep蛋白的作用机制研究较少。因此,本文结合国内外近年研究进展,对PCV2 Rep蛋白的胞内互作蛋白进行了系统分析,尤其对现今已发现的8种胞内互作蛋白在PCV2复制过程中可能发挥的重要作用及机制进行了阐释,并对其他可能与PCV2 Rep蛋白存在相互作用的胞内蛋白进行预测,以期为进一步阐明Rep蛋白在PCV2致病中的作用提供理论依据。Porcine circovirus virus type 2(PCV2)is a major pathogen that causes postweaning multisystemic wasting syndrome(PMWS)and other swine-related diseases in piglets.Replicase(Rep)is a functional replication initiation protein encoded by the ORF1 gene participating in the DNA rolling loop replication of PCV2 by interacting with Rep′,another replication-related protein encoded by ORF.As an essential functional protein of PCV2,Rep could become a new target that can lead to breakthroughs in the research on antiviral therapy for PCV2,as most of the existing studies on the pathogenesis of PCV2 and vaccine have focused on Cap.This article reviewed the latest reports published in the world to systematically analyze Rep,especially,the critical roles and mechanisms that 8 known kinds of intracellular proteins interacted with it may play in the replication of PCV2.Furthermore,other cellular proteins that are possibly associated with the PCV2 Rep but not yet identified by the scientific communities are also included in the discussion for future exploration on the PCV2 pathogenesis.

关 键 词：猪圆环病毒2型 REP蛋白 互作蛋白 宿主蛋白 

分 类 号：S853.65[农业科学—临床兽医学]