有氧运动预适应改善骨髓间充质干细胞治疗急性心肌梗死的效果  

Aerobic exercise preconditioning improves therapeutic effect of bone marrow mesenchymal stem cells on acute myocardial infarction

作　　者：张敏[1] 娄国[2] 付常喜[3] Zhang Min;Lou Guo;Fu Changxi(Nanjing Vocational College of Tourism,Nanjing 211100,Jiangsu Province,China;Jiangsu Vocational Institute of Commerce,Nanjing 211168,Jiangsu Province,China;Department of Physical Education,Xuzhou University of Technology,Xuzhou 221008,Jiangsu Province,China)

机构地区：[1]南京旅游职业学院,江苏省南京市211100 [2]江苏经贸职业技术学院,江苏省南京市211168 [3]徐州工程学院体育学院,江苏省徐州市221008

出　　处：《中国组织工程研究》2024年第25期3988-3993,共6页Chinese Journal of Tissue Engineering Research

基　　金：江苏省社会科学基金项目(22TYD001),项目负责人:付常喜。

摘　　要：背景:干细胞疗法是急性心肌梗死后恢复受损心肌组织的替代治疗策略,运动预适应可诱导机体产生内源性心脏保护效应,然而两者联合应用的疗效及机制尚不清楚。目的:探讨运动预适应联合骨髓间充质干细胞对急性心肌梗死大鼠治疗效果的影响及其可能机制。方法:70只雄性SD大鼠随机分为假手术组、模型组、干细胞治疗组、运动预适应组和联合干预组。运动预适应组和联合干预组于造模前进行8周跑台有氧运动,然后通过结扎冠状动脉前降支制作急性心肌梗死模型,干细胞治疗组和联合干预组于造模后隔天尾静脉注射骨髓间充质干细胞(1×10^(9)L^(-1),1 mL),治疗4周后,利用递增负荷跑台运动实验评估运动能力,超声心动图检测心脏结构与功能;分离左心室,2,3,5-氯化三苯基四氮唑染色评估心肌梗死面积,Masson染色检测胶原容积分数,CD31免疫组织化学染色检测心肌毛细血管密度,TUNEL染色检测心肌细胞凋亡情况,免疫印迹法检测基质细胞衍生因子1、CXC趋化因子受体蛋白4、肿瘤坏死因子α、白细胞介素10和血管内皮生长因子蛋白表达量。结果与结论:①干预疗效:与假手术组比较,模型组运动能力、左心室射血分数、左心室缩短分数、CD31阳性细胞率下降(P<0.05),心肌梗死面积、胶原容积分数和心肌细胞凋亡率增加(P<0.05)。与模型组比较,干细胞治疗组运动能力无显著差异(P>0.05),运动预适应组和联合干预组运动能力提高(P<0.05);干细胞治疗组、运动预适应组、联合干预组左心室射血分数、左心室缩短分数、CD31阳性细胞率升高(P<0.05),心肌梗死面积、胶原容积分数、心肌细胞凋亡率降低(P<0.05)。与干细胞治疗组比较,联合干预组运动能力、左心室射血分数、左心室缩短分数、CD31阳性细胞率增加(P<0.05),心肌梗死面积、胶原容积分数、心肌细胞凋亡率降低(P<0.05)。②蛋白表�BACKGROUND:Stem cell therapy is an alternative treatment strategy for restoring damaged myocardial tissue after acute myocardial infarction.Exercise preconditioning can induce endogenous cardioprotective effects in the body.However,the efficacy and mechanism of the combined application are still unclear.OBJECTIVE:To explore the effect and possible mechanism of exercise preconditioning combined with bone marrow mesenchymal stem cells on the therapeutic effect in rats with acute myocardial infarction.METHODS:Seventy male SD rats were randomly divided into sham operation group,model group,stem cell therapy group,exercise preconditioning group,and combined intervention group.Rats in the exercise preconditioning group and combined intervention group underwent 8-week aerobic exercise on the treadmill before modeling.The animal model of acute myocardial infarction was made by ligating the anterior descending coronary artery.The stem cell therapy group and the combined intervention group were injected with bone marrow mesenchymal stem cells(1×10^(9)L^(-1),1 mL)through the tail vein the next day after modeling.After 4 weeks of treatment,the exercise performance was evaluated by a graded treadmill exercise test.The cardiac structure and function were detected by echocardiography.The left ventricle was isolated.2,3,5-Triphenyltetrazolium chloride staining was used to evaluate myocardial infarct size.Masson staining was used to obtain collagen volume fraction.CD31 immunohistochemical staining was used to detect myocardial capillary density.TUNEL staining was used to detect myocardial cell apoptosis.Immunoblotting was used to detect protein expression levels of stromal cell-derived factor 1,CXC chemokine receptor protein 4,tumor necrosis factor-α,interleukin-10,and vascular endothelial growth factor.RESULTS AND CONCLUSION:(1)Intervention efficacy:Compared with the sham operation group,exercise performance,left ventricular ejection fraction,left ventricular fractional shortening,and CD31 positive cell rate decreased(P<0.05);

关 键 词：运动预适应 急性心肌梗死 骨髓间充质干细胞 归巢 心脏重塑 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] G804.5[文化科学—运动人体科学]