间充质干细胞治疗皮瓣缺血再灌注损伤的作用机制及优势  

Action mechanism and advantages of mesenchymal stem cells for treating flap ischemia-reperfusion injury

作　　者：何波 何志军[2] 刘涛 马岁录 魏晓涛 王威威 He Bo;He Zhijun;Liu Tao;Ma Suilu;Wei Xiaotao;Wang Weiwei(College of Traditional Chinese Medicine,Gansu University of Chinese Medicine,Lanzhou 730030,Gansu Province,China;Department of Foot and Ankle Orthopedics,Gansu Hospital of Traditional Chinese Medicine,Lanzhou 730050,Gansu Province,China)

机构地区：[1]甘肃中医药大学中医临床学院,甘肃省兰州市730030 [2]甘肃省中医院足踝骨科,甘肃省兰州市730050

出　　处：《中国组织工程研究》2024年第25期4065-4071,共7页Chinese Journal of Tissue Engineering Research

基　　金：甘肃省中医药项目(GZKZ-2020-2),项目名称:基于多组学及网络药理学研究消肿止痛合剂治疗皮瓣缺血再灌注损伤的作用机制,项目负责人:何志军;国家自然科学基金(81660802),项目名称:消肿止痛合剂对皮瓣修复后血管再生及其信号通路VEGF-Dll4/Notch的机制研究,项目负责人:何志军。

摘　　要：背景:间充质干细胞因具有抗氧化、抑制炎症反应和诱导血管新生等作用而被用于皮瓣缺血再灌注损伤中。目的:综述间充质干细胞在治疗皮瓣缺血再灌注损伤方面的作用机制和最新进展,为其进一步理论研究和临床合理应用提供依据。方法:检索CNKI中国期刊全文数据库、万方数据库和PubMed数据库收录的相关文献。中文检索词为“间充质干细胞;皮瓣缺血再灌注损伤;条件培养基;外泌体;氧化应激;炎症反应;血管新生”。英文检索词为“mesenchymal stem cells;flap ischemia reperfusion injury;Conditioned medium;exosomes;Oxidative stress;Inflammatory reaction;Angiogenesis”。检索2010年以来相关文献,通过阅读文章剔除研究内容与文章主题关系不大、质量较差及内容陈旧文献,最终纳入74篇文献进行归纳总结。结果与结论:①间充质干细胞在抗氧化、抑制炎性反应及诱导血管新生等方面作用显著,在治疗皮瓣缺血再灌注损伤中蕴含着巨大潜力。②然而,间充质干细胞自身的缺陷及近年来治疗效果的下降使间充质干细胞的发展和应用陷入了瓶颈期,间充质干细胞条件培养基及其外泌体和间充质干细胞可塑性研究应运而生,且治疗效果明显优于单纯使用间充质干细胞。③因此,更全面地了解间充质干细胞治疗皮瓣缺血再灌注损伤的作用机制和最新治疗进展,对于间充质干细胞的研究和皮瓣缺血再灌注损伤的治疗都具有非常重要的意义。BACKGROUND:Mesenchymal stem cells are used in flap ischemia-reperfusion injury due to their antioxidant and inflammatory inhibition,and angiogenesis induction.OBJECTIVE:To review the mechanism and latest treatment progress of mesenchymal stem cells in the treatment of flap ischemia-reperfusion injury,and to provide a basis for further theoretical research and clinical rational application.METHODS:We searched the relevant articles indexed in CNKI,WanFang and PubMed databases.Chinese and English search terms were“mesenchymal stem cells;flap ischemia reperfusion injury;conditioned medium;exosomes;oxidative stress;inflammatory reactions;angiogenesis”.Relevant literature since 2010 was searched,and 74 articles were finally included after excluding the literature that had little to do with the topic of the article,poor quality and outdated content.RESULTS AND CONCLUSION:(1)Mesenchymal stem cells play significant roles in antioxidation,inhibition of inflammation and induction of angiogenesis and have great potential in the treatment of flap ischemia-reperfusion injury.(2)However,the defects of mesenchymal stem cells themselves and the decline of therapeutic effect in recent years have put the development and application of mesenchymal stem cells into a bottleneck period,and the research on the plasticity of mesenchymal stem cells conditioned medium and its exosomes and mesenchymal stem cells came into being,and the therapeutic effect was significantly better than the use of mesenchymal stem cells alone.(3)Therefore,a more comprehensive understanding of the mechanism of action and the latest treatment progress of mesenchymal stem cells in the treatment of flap ischemia-reperfusion injury is of great significance for the research of mesenchymal stem cells and the treatment of flap ischemia-reperfusion injury.

关 键 词：间充质干细胞 皮瓣缺血再灌注损伤 条件培养基 外泌体 氧化应激 炎症反应 血管新生 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R285