基于光声泵浦成像的氧分压测量定量分析  

Quantitative analysis of oxygen partial pressure measure-ments based on photoacoustic pump-probe imaging

作　　者：何霄 肖小舟 何滨 薛平[2] 肖嘉莹 He Xiao;Xiao Xiao-Zhou;He Bin;Xue Ping;Xiao Jia-Ying(Department of Biomedical Engineering,School of Basic Medical Science,Central South University,Changsha 410083,China;State Key Laboratory of Low-Dimensional Quantum Physics,Department of Physics,Tsinghua University,Beijing 100084,China)

机构地区：[1]中南大学基础医学院生物医学工程系,长沙410083 [2]清华大学物理系,低维量子物理国家重点实验室,北京100084

出　　处：《物理学报》2023年第21期342-350,共9页Acta Physica Sinica

基　　金：低维量子物理国家重点实验室开放科研基金项目(批准号:KF202209);湖南省自然科学基金(批准号:2022JJ30756);中南大学创新驱动项目(批准号:2020CX004);深圳市科技创新委员会自由探索基础研究计划(批准号:2021Szvup168)资助的课题。

摘　　要：光声泵浦成像是一种新型的高特异性光声分子影像技术.它可以避免常规光声成像中来自血液等强背景信号的干扰,实现组织中微弱目标分子的探测,并通过对泵浦-探测激光间的延时扫描,获得组织中的氧分压分布.本文采用亚甲基蓝作为分子探针,通过对血红蛋白溶液中氧分压变化的监测,开展了对光声泵浦成像的定量分析研究.本文采用高斯噪声模型,获得了三重态差分信号稳定性随着平均次数变化的规律,并在此基础上对氧分压测量的误差进行了分析.结果表明,在平均次数为200次条件下,氧分压在300—550 mmHg(1 mmHg=133 Pa)区间内,所搭建系统的检测精度优于33 mmHg.本研究将对光声泵浦成像技术的进一步发展和应用起到重要的指导作用.Pump-probe-based photoacoustic imaging is an innovative technique for high-specificity molecular imaging in deep tissues.Compared with conventional photoacoustic imaging,this method effectively eliminates the interference from blood signal and other background signal,enabling the detection of subtle target molecules.Additionally,the manipulating of the time delay between the pump laser and probe laser can facilitate noninvasive mapping of oxygen partial pressure distribution within tissues.To quantify the photoacoustic pumpprobe imaging,we use methylene blue as the molecular probe to monitor changes in oxygen partial pressure within a hemoglobin solution.Utilizing a Gaussian noise model,we investigate the relationship between the stability of the triplet-state difference signal and the average number,and also evaluate the error associated with measuring oxygen partial pressure.The results demonstrate that the detection accuracy of the system is better than 33 mmHg(1 mmHg=133 Pa)in the oxygen partial pressure range of about 300 to 550 mmHg after 200 times of averaging.This research will play a significant role in guiding the further advancement and application of pump-probe-based photoacoustic imaging technology.

关 键 词：光声泵浦成像 氧分压 瞬态三重差分 定量分析 

分 类 号：R318[医药卫生—生物医学工程] TP391.41[医药卫生—基础医学]