康复新液调控巨噬细胞极化防治大鼠急性放射性口腔黏膜炎  

Kangfuxin liquid regulates macrophage polarization to prevent acute radiation-induced oral mucositis in rats

作　　者：董克臣 张萌 梁毅[1] 李松 费新雄 DONG Kechen;ZHANG Meng;LIANG Yi;LI Song;FEI Xinxiong(Department of Oncology of Head and Neck,Huangshi Central Hospital(Affiliated Hospital of Hubei Institute of Technology),Huangshi,Hubei 435200,China)

机构地区：[1]黄石市中心医院(湖北理工学院附属医院)头颈肿瘤内科,湖北黄石435200

出　　处：《安徽医药》2023年第12期2366-2370,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨康复新液对大鼠急性放射性口腔黏膜炎(ROM)的防治效果及对巨噬细胞极化的影响。方法该研究于2021年7月至2022年4月进行,将54只SD大鼠按随机数字表法分成对照组、照射组和给药组,各18只。除对照组外,其余各组均行单次大剂量照射模拟急性ROM。给药组予康复新液,其余两组给予生理盐水。观察照射后大鼠一般情况及颊黏膜病理变化。酶联免疫吸附测定(ELISA)检测颊黏膜组织中白细胞介素(IL)-6、肿瘤坏死因子-α(TNF-α)、转化生长因子-β(TGF-β)和IL-10水平。免疫荧光法检测颊黏膜组织CD86和CD206阳性巨噬细胞。逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法检测颊黏膜中诱导型一氧化氮合酶(iNOS)、精氨酸酶1(Arg1)、共刺激分子CD86和CD206 mRNA表达和蛋白水平。结果与照射组比较,给药组大鼠一般情况得到改善,颊黏膜炎症减轻。对照组、照射组和给药组颊黏膜组织IL-6浓度分别为(0.46±0.04)μg/L、(1.12±0.16)μg/L、(0.74±0.08)μg/L,TNF-α浓度分别为(0.12±0.02)μg/L、(0.68±0.10)μg/L、(0.34±0.06)μg/L,TGF-β浓度分别为(0.32±0.06)μg/L、(0.75±0.12)μg/L、(1.26±0.20)μg/L,IL-10浓度分别为(0.58±0.08)μg/L、(0.96±0.14)μg/L、(1.44±0.21)μg/L;三组CD86阳性巨噬细胞数目分别为0、152.54±6.95、99.51±3.61,CD206阳性细胞数目分别为0、88.26±3.10、129.99±2.61;三组iNOS MRNA相对表达水平分别为(0.00443±0.00025、0.00682±0.00041、0.00594±0.00038)、CD86 mRNA相对表达水平分别为(0.00342±0.00020、0.00584±0.00043、0.00422±0.00035),Arg1 mRNA相对表达水平分别为(0.00424±0.00016、0.00520±0.00055、0.00661±0.00053),CD206 mRNA相对表达水平分别为(0.00423±0.00038、0.00682±0.00056、0.00824±0.00086)。康复新液能够减轻急性ROM大鼠颊黏膜炎症反应,抑制M1型巨噬细胞极化,促进M2型巨噬细胞极化,抑制促炎因子在大鼠颊黏膜组织的表达,促进抗炎因子的释放,差异有统计Objective To explore the protective effect of kangfuxin liquid on acute radiation-induced oral mucositis(ROM)in rats and its impact on macrophage polarization.Methods This study was conducted from July 2021 to April 2022.Totally 54 SD rats were randomized into control group,radiation group and administration group,with 18 rats in each group.Except for the control group,the other two groups were irradiated at a single high-dose to make acute ROM model.The administration group was given kangfuxin liquid,while radiation group and control group were given saline solution.The general conditions and histopathological changes of buccal mucosa were observed after irradiation.The levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),tumor growth factor-β(TGF-β),and interleukin-10(IL-10)of the mucosal tissues were measured by enzyme-linked immunosorbent assay(ELISA).CD86 and CD206 positive macrophages in buccal mucosa were detected by immunofluorescence.The mRNA expressions and protein levels of inducible nitric oxide synthase(iNOS),arginase 1(Arg1),costimulatory molecules CD86 and CD206 in buccal mucosa were detected by reverse transcription-polymerase chain reaction(RT-PCR)and Western blotting,respectively.Results Compared with radiation group,the general condition of rats in administration group was improved,and the inflammation of mucosal tissue was alleviated.The concentrations(μg/L)of IL-6,TNF-α,TGF-β,and IL-10 in the control,radiation and administration groups were[(0.46±0.04)μg/L,(1.12±0.16)μg/L,(0.74±0.08)μg/L];[(0.12±0.02)μg/L,(0.68±0.10)μg/L,(0.34±0.06)μg/L];[(0.32±0.06)μg/L,(0.75±0.12)μg/L,(1.26±0.20)μg/L];[(0.58±0.08)μg/L,(0.96±0.14)μg/L,(1.44±0.21)μg/L],respectively.The numbers of CD86 positive macrophages in each group were(0,152.54±6.95,99.51±3.61)respectively;and the numbers of CD206 positive macrophages in each group were 0,88.26±3.10,129.99±2.61,respectively.The relative mRNA expression of iNOS,CD86,Arg1,and CD206 were(0.00443±0.00025,0.00682±0.00041,0.00594±0

关 键 词：黏膜炎 康复新液 大鼠 Sprague-Dawley 转化生长因子-β 放射性口腔黏膜炎 巨噬细胞 极化 

分 类 号：R285.5[医药卫生—中药学]