新生儿支气管肺发育不良病儿外周血信号转导及转录活化因子3、内皮素-1表达及临床意义  

Expression and clinical significance of peripheral blood STAT3 and endothelin-1 in children with neonatal bronchopulmonary dysplasia

作　　者：王彤[1] 张芳[1] 杨国颖[1] WANG Tong;ZHANG Fang;YANG Guoying(Department of Neonatology,Tangshan Maternal and Child Health Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]唐山市妇幼保健院新生儿科,河北唐山063000

出　　处：《安徽医药》2023年第12期2475-2478,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探究新生儿支气管肺发育不良(BPD)病儿外周血信号转导及转录活化因子3(STAT3)、内皮素-1表达水平及临床意义。方法选取2019年3月至2021年3月唐山市妇幼保健院新生儿科收治的93例早产儿为研究对象,根据BPD诊治标准分为BPD组病儿51例,非BPD组病儿42例。收集所有病儿的一般临床资料并分析。采用酶联免疫吸附测定(ELISA)检测病儿不同时期(出生后第1天、第14天、第28天)的血清STAT3、内皮素-1水平。受试者操作特征(ROC)曲线分析血清STAT3、内皮素-1对BPD的诊断价值。多因素logistic分析新生儿发生BPD的危险因素。结果BPD组与非BPD组病儿性别、产妇产前感染、院内感染之间的差异无统计学意义(P>0.05),胎龄、出生体质量、合并肺炎、合并贫血、呼吸暂停、宫内感染、动脉导管未闭、机械通气时间之间差异有统计学意义(P<0.05)。组间比较:BPD组病儿血清STAT3、内皮素-1水平第1天[(1.92±0.54)ng/L、(108.74±21.64)ng/L]、第14天[(1.43±0.37)ng/L、(83.16±16.34)ng/L]、第28天[(1.01±0.19)ng/L、(59.69±9.46)ng/L]均高于非BPD组[(1.16±0.25)ng/L、(65.16±10.62)ng/L,(0.98±0.18)ng/L、(54.34±9.11)ng/L,(0.80±0.16)ng/L、(46.78±7.84)ng/L],差异有统计学意义(P<0.05);组内比较:第14天、第28天两组病儿血清STAT3、内皮素-1水平均低于第1天,第28天两组病儿血清STAT3、内皮素-1水平均低于第14天,差异有统计学意义(P<0.05)。ROC曲线分析显示,血清STAT3、内皮素-1对BPD诊断价值的曲线下面积(AUC)分别为0.83、0.85,截断值分别为1.65 ng/L、73.57 ng/L,灵敏度分别为60.80%、92.20%,特异度分别为95.20%、81.00%。多因素logistic回归分析显示合并肺炎、机械通气时间≥7 d及高水平的STAT3和内皮素-1是新生儿发生BPD的独立危险因素(P<0.05),而出生体质量1000~1250 g、>1250~<1500 g是新生儿发生BPD的保护因素(P<0.05)。结论BPD病儿血清STAT3、内皮素-1水平呈异常表达,且对Objective To explore the expression levels and clinical significance of peripheral blood signal transducer and activator of transcription 3(STAT3)and endothelin-1 in children with neonatal bronchopulmonary dysplasia(BPD).Methods Ninety-three premature infants admitted to the Department of Neonatology,Tangshan Maternal and Child Health Hospital from March 2019 to March 2021 were selected as the study subjects.According to the diagnosis and treatment criteria of BPD,51 cases were divided into the BPD group,and 42 cases were divided into the non-BPD group.General clinical data of all infants were collected and analyzed.Serum STAT3 and endothelin-1 levels were detected by enzyme-linked immunosorbent assay(ELISA)at different periods(postnatal day 1,day 14 and day 28).The diagnostic value of serum STAT3 and endothelin-1 for BPD was analyzed by receiver operating characteristic(ROC)curves.Multivariate logistic analysis of risk factors for the development of BPD in neonates.Results There were no statistically significant differences between the BPD group and the non-BPD group in terms of sex,prenatal and nosocomial infection of the mothers(P>0.05),while there were statistically significant differences in terms of gestational age,birth weight,copneumonia,coanemia,apnea,intrauterine infection,patent ductus arteriosus,and mechanical ventilation time(P<0.05).Comparison between groups:serum STAT3 and endothelin-1 levels in children in the BPD group were[(1.92±0.54)ng/L and(108.74±21.64)ng/L]on day 1,[(1.43±0.37)ng/L and(83.16±16.34)ng/L]on day 14,and[(1.01±0.19)ng/L and(59.69±9.46)ng/L]on day 28 L,which were higher than those in the non-BPD group[(1.16±0.25)ng/L,(65.16±10.62)ng/L,(0.98±0.18)ng/L,(54.34±9.11)ng/L,(0.80±0.16)ng/L,(46.78±7.84)ng/L],and the differences were statistically significant(P<0.05).Intragroup comparison:serum STAT3 and endothelin-1 levels were lower in both groups on days 14 and 28 than on day 1,serum STAT3 and endothelin-1 levels were lower in both groups on day 28 than on day 14,and the dif

关 键 词：支气管肺发育不良 信号转导及转录活化因子3 内皮素-1 身体质量指数 婴儿 新生 

分 类 号：R722.1[医药卫生—儿科]