HGF/c-Met信号通路与小鼠早期卵泡发育的相关性研究  

Role of hepatocyte growth factor(HGF)/c-Met signaling pathway in early follicular development in mice

作　　者：黎明星 李卓娴 李康梅 陈秀榕 周艳平 黄愉淋 白琳 吴展帅 夏猛 何国珍 LI Mingxing;LI Zhuoxian;LI Kangmei;CHEN Xiurong;ZHOU Yanping;HUANG Yulin;BAI Lin;WU Zhanshuai;XIA Meng;HE Guozhen(Basic Medical College,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine,Nanning 530200,China;不详)

机构地区：[1]广西中医药大学基础医学院,南宁530200 [2]广西高发传染病中西医结合转化医学重点实验室,南宁530200

出　　处：《实用医学杂志》2023年第20期2566-2571,共6页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81960794);广西自然科学基金项目(编号:2020GXNSFBA159064);广西中青年教师科研基础能力提升项目(编号:2019KY0320,2021KY0320);广西中医药大学引进博士科研启动基金项目(编号:2018BS007,2018BS015);广西中医药大学高层次人才培育创新团队资助项目(编号:2022B006);广西中医药大学第二批“岐黄工程”高层次人才团队培育项目(编号:2021001);广西中医药大学大学生创新创业训练计划项目(编号:S202110600092);广西中医药大学大学生科研训练课题(编号:2022DXS04)。

摘　　要：目的通过检测肝细胞生长因子(HGF)及其受体(c-Met)在早期小鼠卵巢组织的表达情况,探讨HGF/c-Met信号通路在小鼠早期卵泡发育的相关性,并通过生物信息学分析HGF/c-Met通路的生物学过程及涉及的细胞内信号通路,为后续研究HGF/c-Met通路调节早期卵泡发育的分子机制打下基础。方法选用昆明小鼠以雌∶雄(2∶1)合笼,收集生后3、7、14、21 d卵巢,采用免疫组学、蛋白免疫印迹分别检测卵巢HGF和c-Met蛋白表达及表达水平;实时荧光定量PCR检测卵巢HGF、c-Met的mRNA表达水平。结果HGF及c-Met蛋白在3、7、14、21 d卵巢中均有表达,且表达量随着小鼠日龄增长而增加(P<0.05),HGF、c-Met的m RNA水平也呈现相同的趋势(P<0.05);生物信息学分析发现HGF/c-Met通路涉及了多种生物学过程及PI3K/AKT等信号通路。结论HGF/c-Met通路参与小鼠早期卵泡发育,并可能通过细胞内信号通路PI3K/AKT调控原始卵泡启动及卵泡发育的过程。Objective To investigate the role of hepatocyte growth factor and its receptor(c-Met)(HGF/c-Met)signaling pathway in early follicular development in mice via detecting the expression of HGF/c-Met in early mouse ovarian tissue and then analyze the biological process of HGF/c-Met signaling pathway and its possible involvement in intracellular signaling pathways by using bioinformatics for the purpose of further studying the molecular mechanism of the signaling pathway in the early follicle development.Methods Thirty-five female Kunming mice and 15 male ones were fed in 15 cages with the female-to-male ratio of 2∶1.The ovaries of mice at 3,7,14 and 21 days after birth were collected.Immunohistochemical staining and Western blotting were applied to detect the expression levels of HGF and c-Met protein in the ovarian tissues.Quantitative Real-time PCR(q-PCR)was used to detect the expression levels of HGF and C-Met mRNA in the early ovarian tissues of mice.Results HGF and c-Met proteins were expressed in the ovarian tissues of the mice at 3,7,14 and 21 days,presenting an increasing trend with the increase of age by day(P<0.05).The mRNA levels of HGF and c-Met also showed the same trend(P<0.05).Bioinformatics analysis revealed that the HGF/c-Met signaling pathway involved in a variety of biological processes and signaling pathways such as PI3K/AKT.Conclusions HGF/c-Met signaling pathway is involved in early follicular development in mice.It may regulate primal follicular initiation and follicular development through intracellular signaling pathway PI3K/AKT.

关 键 词：肝细胞生长因子 肝细胞生长因子受体 早期卵泡发育 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]