重复经颅磁刺激对慢性抑郁小鼠前额叶皮质和海马区P2X7R及GFAP表达的影响  

The effects of transcranial magnetic stimulation on the expression of P2X7R and GFAP in the prefrontal cortex and hippocampus of mice modeling chronic depression

作　　者：曾毅[1] 周丽[2] 马艳[2] 余玲玲 孙瑞[2] Zeng Yi;Zhou Li;Ma Yan;Yu Lingling;SunRui(Department of Hospital Infection Management,Wuhan No.1 Hospital,Wuhan 430022,China;Department of Rehabilitation,Wuhan No.1 Hospital,Wuhan 430022,China;Institute of Integrated Traditional Chinese and Western Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]武汉市第一医院医院感染办公室,武汉430022 [2]武汉市第一医院康复科,武汉430022 [3]华中科技大学同济医学院附属同济医院中西医结合研究所,武汉430030

出　　处：《中华物理医学与康复杂志》2023年第2期97-102,共6页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：湖北省知识创新专项(2019CFC921);湖北省卫生健康委员会中医药科研项目(ZY2021M045)。

摘　　要：目的观察重复经颅磁刺激(rTMS)对抑郁小鼠前额叶皮质和海马区嘌呤2X7受体(P2X7R)及胶质纤维酸性蛋白(GFAP)表达的影响。方法将30只C57BL/6小鼠分为对照组(10只)和造模组(20只)。对照组小鼠群居饲养(5只/笼),造模组小鼠在出生21 d后通过断奶独居(1只/笼)饲养6周制作慢性抑郁小鼠模型。采用随机数字表法将16只制模成功小鼠分为模型组及rTMS组,每组8只小鼠。rTMS组小鼠给予10 Hz rTMS干预,每周干预5 d。于rTMS干预4周后观察各组小鼠抑郁样行为改变,并对比各组小鼠前额叶皮质和海马区P2X7R、GFAP表达变化。结果与对照组比较,模型组小鼠蔗糖偏好实验(SPT)中糖水偏好量、旷场实验(OFT)中运动距离均显著减少(P<0.05),悬尾实验(TST)中静止不动时间显著增加(P<0.05),前额叶皮质和海马区P2X7R表达水平均明显增加(P<0.05),GFAP表达水平则显著下降(P<0.05)。与模型组比较,rTMS组SPT糖水偏好量[(75.11±4.58)%vs(65.14±4.87)%]、OFT运动距离[(2289.34±100.16)cm vs(2028.90±178.21)cm]均显著增加(P<0.05),TST静止不动时间[(78.11±10.89)s vs(101.39±10.38)s]明显缩短(P<0.05),前额叶皮质和海马区P2X7R表达均明显降低(P<0.05),GFAP表达则显著增强(P<0.05)。结论rTMS干预能有效改善早期独居小鼠抑郁状态,其治疗机制可能与抑制前额叶皮质和海马区P2X7R表达、促进GFAP表达有关。Objective To investigate any effect of repeated transcranial magnetic stimulation(rTMS)on the expression of P2X7 receptor(P2X7R)and glial fibrillary acid protein(GFAP)in the prefrontal cortex and hippocampus of mice modeling depression.Methods Thirty C57BL/6 mice were divided into a control group(n=10)and a depression group(n=20).The mice of the control group were raised in group(five mice per cage),while those of the depression group were kept alone for six weeks to induce depression.Among them,16 were successfully modeled and randomly divided into a model group(n=8)and an rTMS group(n=8).The rTMS group received five sessions per week of 10Hz rTMS for 4 weeks.Any changes in depression-like behavior were observed and the expression of P2X7R and GFAP in the prefrontal cortex and hippocampus was measured.Results Compared to the control group,a significant decrease was observed in the sucrose consumption rate in the sucrose preference test,in the distance moved in the open field test and in the expression of GFAP protein.But there was a significant increase in the immobile time in the tail suspension test and in the expression of P2X7R protein in the prefrontal cortex and hippocampus in the model group.At the conclusion of the experiment the differences in the sucrose consumption rate,the distance moved,GFAP protein expression,immobile time and P2X7R protein expression between the rTMS and the model group were all statistically significant.Conclusion rTMS can reduce depression-like behavior,at least in mice.That may be related to inhibiting P2X7R expression and promoting GFAP expression in the prefrontal cortex and hippocampus.

关 键 词：重复经颅磁刺激 抑郁症 嘌呤2X7受体 胶质纤维酸性蛋白 社会隔离 

分 类 号：R749.4[医药卫生—神经病学与精神病学]