流体剪切力通过下调p21促进MC3T3-E1成骨细胞增殖  

Fluid Shear Stress Promotes Proliferation of MC3T3-E1 Osteoblasts Through Down-Regulating p21

作　　者：移穷 刘众成 刘雪宁 张坤 王力夫 耿彬[1] 夏亚一[1] YI Qiong;LIU Zhongcheng;LIU Xuening;ZHANG Kun;WANG Lifu;GENG Bin;XIA Yayi(Department of Orthopedics,Lanzhou University Second Hospital/Orthopedic Clinical Medical Research Center of Gansu Province/Orthopedics Key Laboratory of Gansu Province,Lanzhou 730000,China)

机构地区：[1]兰州大学第二医院骨科、甘肃省骨科临床医学研究中心、甘肃省骨关节疾病研究重点实验室,兰州730030

出　　处：《医用生物力学》2023年第4期663-669,682,共8页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(81874017,81960403,82060405);兰州市科技计划项目(2021-RC-102);兰州大学第二医院“萃英科技创新”计划(CY2017-ZD02,CY2021-MS-A07)。

摘　　要：目的探讨流体剪切力(fluid shear stress,FSS)对成骨细胞p21表达的影响,并明确p21在FSS诱导的成骨细胞增殖过程中的作用。方法对成骨细胞加载不同时间(0、15、30、45、60 min)、1.2 Pa FSS。用CCK-8实验、EdU实验检测成骨细胞增殖活性。用siRNA p21或pcDNA p21转染成骨细胞,并用Western blotting检测转染效果。Western blotting检测不同干预条件下p21、cyclin D1、CDK4的表达变化。结果加载1.2 Pa FSS后,p21表达显著下调,且加载45 min后表达水平最低。加载FSS和下调p21表达都显著增强成骨细胞增殖,并增加cyclin D1、CDK4表达。而上调p21表达后,加载FSS不再具有增强成骨细胞增殖和增加cyclin D1、CDK4表达的作用。结论1.2 Pa FSS能够下调成骨细胞p21表达,在加载45 min时下调最为明显。p21下调对成骨细胞增殖具有促进作用,且FSS通过下调p21促进成骨细胞增殖。Objective To investigate the effect of fluid shear stress(FSS)on p21 expression in osteoblasts,and to clarify the role of p21 in osteoblast proliferation induced by FSS.Methods Osteoblasts were treated with 1.2 Pa FSS for different duration(0,15,30,45,60 min).Osteoblasts were transfected with siRNAp21 or pcDNAp21,and the transfection effect was detected by Western blotting.The proliferation of osteoblasts was assessed by CCK-8 assay or EdU labelling assay.The expression of p21,cyclinD1 and CDK4 was detected by Western blotting.Results 1.2 Pa FSS significantly down-regulated p21 expression,and the expression level of p21 was the lowest when treated with FSS for 45 min.Both FSS and down-regulation of p21 significantly increased the proliferation of osteoblasts and the expression of cyclin D1 and CDK4.However,after up-regulating p21 expression,FSS couldn’t increase the proliferation of osteoblasts and the expression of cyclin D1 and CDK4.Conclusions 1.2 Pa FSS can down-regulate p21 expression in osteoblasts,and the expression level of p21 was the lowest when treated with FSS for 45 min.Down-regulation of p21 can promote osteoblast proliferation,and FSS promotes osteoblast proliferation through down-regulating p21.

关 键 词：流体剪切力 成骨细胞 细胞增殖 

分 类 号：R318.01[医药卫生—生物医学工程]