硫代硫酸盐投加对含硫废水中硫细菌与聚糖菌竞争的短期影响  

SHORT-TERM EFFECT OF THIOSULFATE ON COMPETITION BETWEEN SULFUR BACTERIA AND GLYCOGEN ACCUMULATING ORGANISMS IN SULFUR-CONTAINING WASTEWATER

作　　者：周立昌 李昭陵 陈磊 林亚楠 龚志伟 林青山 马杰 王宗平[1] 郭刚 ZHOU Lichang;LI Zhaoling;CHEN Lei;LIN Ya'nan;GONG Zhiwei;LIN Qingshan;MA Jie;WANG Zongping;GUO Gang(School of Environmental Science and Engineering,Huazhong University of Science and Technology,Wuhan 430074,China;East China Electric Power Design Institute,CPECC,Shanghai 200333,China;Wuhan Municipal Engineering Design&Research Institute Co.,Ltd.,Wuhan 430023,China;China Railway Fourth Survey and Design Institute Group Co.,Ltd.,Wuhan 430063,China)

机构地区：[1]华中科技大学环境科学与工程学院,武汉430074 [2]中国电力工程顾问集团华东电力设计院有限公司,上海200333 [3]武汉市政工程设计研究院有限责任公司,武汉430023 [4]中铁第四勘察设计院集团有限公司,武汉430063

出　　处：《环境工程》2023年第8期26-32,共7页Environmental Engineering

基　　金：国家自然科学基金“单质硫强化低碳源污水反硝化除磷机理及调控”(52100040);湖北省重点研发计划项目“工业含盐废水脱盐、重金属去除及深度脱碳关键技术研究”(2022BCA065);国家重点研发计划项目“长江经济带大中城市多源有机固废园区协同处置及示范”(2019YFC1904005)。

摘　　要：硫循环协同反硝化生物除磷工艺的主要功能菌群——硫细菌(包括硫酸盐还原菌和硫氧化菌)易受聚糖菌竞争而导致除磷效果波动。硫代硫酸盐具有较高的生物可利用性,在含硫废水处理中应用广泛。为探究硫代硫酸盐对硫细菌与聚糖菌竞争的短期影响,向已富集硫细菌与聚糖菌的系统中投加不同浓度的硫代硫酸盐[20(R1)、50(R2)和80 mg/L(R3)(以S计)],监测反应器内关键离子和细菌胞内聚合物变化。结果表明:当硫代硫酸盐投加量从20增加到50 mg/L时,反应器缺氧阶段硫酸盐生成量和聚硫降解量分别提高了25%和11%,聚羟基脂肪酸脂(PHA)降解量、糖原生成量相应分别减少了1.60,0.94 mmol/g;而投加量进一步增加到80 mg S/L时,R3较R2聚硫降解量下降了37%,PHA降解量和糖原生成量分别增加了1.08,0.12 mmol C/g VSS。结果表明,投加适量的硫代硫酸盐(50 mg S/L)时,硫氧化菌在与聚糖菌竞争电子供体时会优先利用硫代硫酸盐,有利于提高硫氧化菌的竞争优势。同时,缺氧阶段化学反应动力学结果表明,硫代硫酸盐投加可以提高硫细菌的硫酸盐生成和反硝化速率,从而促进硫细菌的活性。虽然硫代硫酸盐投加可以使反应器保持较好的脱氮效果,但对磷去除效能提升并不明显。Sulfur bacteria,including sulfate-reducing bacteria and sulfur-oxidizing bacteria,as the main functional bacteria of the denitrifying sulfur conversion-associated enhanced biological phosphorus removal(DS-EBPR)process,is susceptible to the competition with glycogen accumulating organisms,resulting in fluctuations in phosphorus removal efficiency.Thiosulfate has high bioavailability and is widely used in the treatment of sulfur-containing wastewater.To explore the short-term effect of thiosulfate on the competition between sulfur bacteria and glycogen-accumulating organisms,different concentrations of thiosulfate[20(R1),50(R2)and 80 mg S/L(R3)]were added to the system enriched with sulfur bacteria and glycogen accumulating organisms to monitor the changes of key ions and bacterial intracellular polymers in the reactors.The results showed that when the dosage of thiosulfate increased from 20 to 50 mg S/L,the amount of sulfate production and polysulfide degradation in the anoxic stage increased by 25%and 11%,respectively,and the amount of PHA degradation,glycogen production decreased by 1.60 and 0.94 mmol C/g VSS,respectively;when the dosage further increased to 80 mg S/L,the degradation of polysulfide of R3 decreased by 37%,and the amount of PHA degradation and glycogen production increased by 1.08 and 0.12 mmol C/g VSS,respectively,compared with that of R2.The results showed that when an appropriate amount of thiosulfate(50 mg S/L)was added,sulfur-oxidizing bacteria would preferentially utilize thiosulfate for electron donors when they competed with glycogen accumulating organisms,which was beneficial to enhancing the competitive advantage of sulfur-oxidizing bacteria.At the same time,the results of chemical reaction kinetics in the anoxic stage showed that the addition of thiosulfate could increase the sulfate production and denitrification rate of sulfur bacteria,thereby promoting the activity of sulfur bacteria.Although the addition of thiosulfate could maintain a good denitrification effect in the reactors,it

关 键 词：含硫废水 硫细菌 聚糖菌 硫代硫酸盐 竞争 

分 类 号：X703[环境科学与工程—环境工程] X172