有机物浓度变化对复合式SBR厌氧氨氧化系统脱氮性能及菌群结构的影响  

EFFECT OF ORGANIC MATTER CONCENTRATION VARIATION ON NITROGEN REMOVAL PERFORMANCE AND BACTERIA COMMUNITY STRUCTURE IN A HYBRID SBR ANAMMOX SYSTEM

作　　者：门艳 刘灵婕 朱雅新 毕艳孟 孟凡盛 于静洁 王少坡[1,2] MEN Yan;LIU Lingjie;ZHU Yaxin;BI Yanmeng;MENG Fansheng;YU Jingjie;WANG Shaopo(School of Environmental and Municipal Engineering,Tianjin Chengjian University,Tianjin 300384,China;Tianjin Key Laboratory of Aquatic Science and Technology,Tianjin 300384,China)

机构地区：[1]天津城建大学环境与市政工程学院,天津300384 [2]天津市水质科学与技术重点实验室,天津300384

出　　处：《环境工程》2023年第8期83-90,共8页Environmental Engineering

基　　金：天津市自然科学基金资助项目(18JCZDJC10080)。

摘　　要：采用序批式反应器(SBR)考察有机物(COD)浓度对复合式厌氧氨氧化(Anammox)反应系统脱氮性能及微生物群落结构的影响。结果表明:在进水ρ(COD)为120 mg/L条件下,系统强化脱氮效果最佳,平均总氮去除效率(NRE)和总氮去除率(NRR)分别为95.4%和0.40 kg/(m^(3)·d);当进水ρ(COD)继续增至150 mg/L时,Anammox活性明显下降,NRE和NRR分别降至75.8%和0.32 kg/(m^(3)·d)。16S rRNA高通量测序结果表明,高COD(120~150 mg/L)下,反应体系内反硝化菌相对丰度明显增加,反硝化菌主要存在于絮状污泥中,而絮状污泥中几乎检测不到Anammox菌,Anammox菌在生物膜上则保持着较高的相对丰度(19.1%~32.5%)。这说明适宜的COD浓度可使Anammox与反硝化作用有效耦合,实现SBR系统脱氮功能强化,且反应体系内生物膜的存在是保障复合式Anammox系统稳定脱氮的重要因素。A sequencing batch reactor(SBR)was used to investigate the effect of organic matter(COD)concentration on nitrogen removal performance and microbial community structure of a hybrid anammox system.The results showed that under the condition of influent COD concentration of 120 mg/L,the system had the best-enhanced nitrogen removal efficiency,and the average total nitrogen removal efficiency(NRE)and total nitrogen removal rate(NRR)were 95.4%and 0.40 kg/(m^(3)·d),respectively;when the influent COD continued to increase to 150 mg/L,the anammox activity decreased obviously,and the NRE and NRR decreased to 75.8%and 0.32 kg/(m^(3)·d),respectively.The results of 16S rRNA high-throughput sequencing showed that the relative abundance of denitrifying bacteria in the system increased significantly at a high COD concentration(120~150 mg/L),and denitrifying bacteria were mainly found in the flocculated sludge,while the Anammox bacteria were almost undetectable in the flocculated sludge,and Anammox bacteria maintained a high relative abundance(19.1%~32.5%)on the biofilm.This indicated that an appropriate COD concentration could achieve the couple of Anammox with denitrification and effectively enhance the function of the SBR system,and the presence of biofilm in the system ensured the nitrogen removal stability of the hybrid Anammox system.

关 键 词：厌氧氨氧化 反硝化 生物脱氮 絮状污泥 生物膜 

分 类 号：X703[环境科学与工程—环境工程] X172