Spontaneous movement of a retrotransposon generated genic dominant male sterility providing a useful tool for rice breeding  

作　　者：Conghao Xu Yifeng Xu Zhengji Wang Xiaoyu Zhang Yuying Wu Xinyan Lu Hongwei Sun Lei Wang Qinglu Zhang Qinghua Zhang Xianghua Li Jinghua Xiao Xu Li Mingfu Zhao Yidan Ouyang Xianbo Huang Qifa Zhang 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan),Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,China [2]Ningde Inspection and Testing Centre for Agricultural Product Quality and Safety,Ningde 352100,China [3]Fujian Academy of Agricultural Sciences,Fuzhou 350018,China [4]Sanming Institute of Agricultural Sciences,Shaxian 365509,China

出　　处：《National Science Review》2023年第9期198-207,共10页国家科学评论（英文版）

基　　金：supported by the National Natural Science Foun-dation of China(31991223,91940301,31991222 and 31821005);the National Key Research and Development Program of China(2022YFF1002100);the Hubei Key R&D Program in Hongshan Lab(2021hszd005 and 2022hszd017);the Fundamental Re-search Funds for the Central Universities(2662023PY002).

摘　　要：Male sterility in plants provides valuable breeding tools in germplasm innovation and hybrid crop production.However,genetic resources for dominant genic male sterility,which hold great promise to facilitate breeding processes,are extremely rare in natural germplasm.Here we characterized the Sanming Dominant Genic Male Sterility in rice and identified the gene SDGMS using a map-based cloning approach.We found that spontaneous movement of a 1978-bp long terminal repeat(LTR)retrotransposon into the promoter region of the SDGMS gene activates its expression in anther tapetum,which causes abnormal programmed cell death of tapetal cells resulting in dominant male sterility.SDGMS encodes a ribosome inactivating protein showing N-glycosidase activity.The activation of SDGMS triggers transcription reprogramming of genes responsive to biotic stress leading to a hypersensitive response which causes sterility.The results demonstrate that an ectopic gene activation by transposon movement can give birth to a novel trait which enriches phenotypic diversity with practical utility.

关 键 词：Oryza sativa dominant male sterility ribosome-inactivating protein RETROTRANSPOSON 

分 类 号：S511[农业科学—作物学]