黄芪多糖对急性肝内胆汁淤积性肝损伤大鼠模型的影响  

Effect of Astragalus Polysaccharides on Acute Intrahepatic Cholestasis Liver Injury Rat Model

作　　者：牛美兰[1] 雷永浩 张郡核 王子昂 王梓淇 邵琳涵 王翔龙 Niu Meilan;Lei Yonghao;Zhang Junhe;Wang Ziang;Wang Ziqi;Shao Linhan;Wang Xianglong(School of Medicine,Huanghe S&T College,Zhengzhou City,Henan Province 450063)

机构地区：[1]黄河科技学院医学院,河南郑州450063

出　　处：《黄河科技学院学报》2023年第11期1-5,共5页Journal of Huanghe S&T College

基　　金：河南省高校省级大学生创新创业训练计划项目(202211834069)。

摘　　要：探究黄芪多糖对急性肝内胆汁淤积性肝损伤大鼠血清丙氨酸氨基转移酶(alanine transaminase,ALT)、天冬氨酸氨基转移酶(Aspartate transaminase,AST)、肿瘤坏死因子a(tumornecrosisfactor-a,TNF-a)、白细胞介素6(interleukin6,IL-6)水平的影响。将雄性健康SD大鼠54只随机分为空白组、实验组(模型组、熊去氧胆酸组及黄芪多糖低、中、高剂量组),每组9只。除空白组、模型组外,其余四组大鼠均给予熊去氧胆酸溶液及黄芪多糖低、中、高(0.2、0.4、0.8 g/kg)溶液,1次/d,共7 d。于第5 d,实验组给予1%α-萘异硫氰酸酯(ANIT)油溶液灌胃建立模型,空白组给予等容积橄榄油。观察大鼠一般情况,采用全自动生化分析仪检测血清ALT、AST含量,采用ELISA法检测血清中炎性因子TNF-α、IL-6表达水平。与空白组相比,模型组ALT、AST、TNF-α、IL-6均升高,具有显著性差异(P<0.01)。与模型组相比,熊去氧胆酸组及黄芪多糖低剂量组、中剂量组、高剂量组均降低,具有显著性差异(P<0.01)。与熊去氧胆酸组相比,黄芪高剂量组效果最为显著,ALT、AST、TNF-α、IL-6均降低,具有显著性差异(P<0.01)。黄芪多糖可以缓解ANIT诱导的急性肝内胆汁淤积性肝损伤并降低ALT、AST、TNF-α、IL-6水平。To investigate the effects of astragalus polysaccharides on serum alanine transaminase(ALT),aspartate transaminase(AST),tumor necrosis factor-a(TNF-a)and interleukin-6(IL-6)levels in rats with acute intrahepatic cholestasis liver injury.54 healthy male SD rats were randomly divided into blank group,experimental group(model group,ursodeoxycholic acid group,astragalus polysaccharide low,medium and high dose group),9 rats in each group.In addition to the blank group and the model group,the other four groups of rats were given ursodeoxycholic acid solution,astragalus polysaccharide low,medium and high(0.2,0.4,0.8 g/kg)solution,once a day for 7 days.On the fifth day,the experimental group was given 1%α-naphthyl isothiocyanate(ANIT)oil solution to establish the model,and the blank group was given equal volume of olive oil.The general condition of rats was observed.The contents of ALT and AST in serum were detected by automatic biochemical analyzer.The expression levels of inflammatory factors TNF-αand IL-6 in serum were detected by ELISA.Compared with the blank group,the model group ALT,AST,TNF-α,IL-6 were increased,with significant difference(P<0.01).Compared with the model group,ursodeoxycholic acid group,astragalus polysaccharide low dose group,middle dose group,high dose group were decreased,with significant difference(P<0.01).Compared with the ursodeoxycholic acid group,the effect of the high-dose Astragalus group was the most significant ALT,AST,TNF-α,and IL-6 decreased,with significant differences(P<0.01).Astragalus polysaccharide can alleviate ANIT-induced acute intrahepatic cholestasis liver injury and reduce ALT,AST,TNF-αand IL-6 levels.

关 键 词：黄芪多糖 Α-萘异硫氰酸酯 急性肝内胆汁淤积性肝损伤 炎症因子 

分 类 号：R445.9[医药卫生—影像医学与核医学]