B(A)亚型2例的血清学及分子生物学鉴定  

Serological and molecular biological identification of 2 cases with B(A)subtype

作　　者：杜振军 邹陆辰 孙恩涛 Du Zhenjun;Zou Luchen;Sun Entao(Liaocheng Central Blood Station,Liaocheng 252000,Shandong Province,China;Department of Health Inspection and Quarantine,Wannan Medical College,Wuhu 241002,Anhui Province,China)

机构地区：[1]聊城市中心血站,聊城252000 [2]皖南医学院检验学院卫生检验与检疫学教研室,芜湖241002

出　　处：《国际输血及血液学杂志》2023年第4期352-357,共6页International Journal of Blood Transfusion and Hematology

摘　　要：目的探讨B(A)亚型个体的血型血清学及分子生物学鉴定方法。方法选择2012年12月15日和2023年2月16日,因血型分型困难,送聊城市中心血站进行血型鉴定的2例受试者为研究对象。受试者1为女性、27岁;受试者2为男性,40岁。采用试管法对受试者的ABO血型进行血型血清学鉴定;采用PCR-序列特异性引物(SSP)法对受试者的ABO基因进行分型;采用Sanger测序法对受试者ABO基因的第6、7外显子进行测序。本研究遵循的程序符合2013年修订的《世界医学协会赫尔辛基宣言》要求。结果①受试者1血清学表型符合AwB或B(A)亚型,受试者2血清学表型符合A2B或B(A)亚型。②受试者1的ABO基因为B(A)02和O杂合,第7外显子存在关键核苷酸杂合突变c.700C>G,其ABO基因分型为B(A).02/O.01.01。受试者2的ABO基因为B(A)04和O杂合,第7外显子存在关键核苷酸杂合突变c.640A>G,其ABO基因分型为B(A).04/O.01.01。结论仅采用血型血清学试验鉴定B(A)亚型困难,ABO基因测序可以为血清血清学试验结果提供分子生物学依据。Objective To explore blood group serological and molecular biological identification methods of B(A)subtype individuals.Methods On December 15,2012 and February 16,2023,a total of 2 subjects who were sent to Liaocheng Central Blood Station for blood group identification due to difficulty in blood group typing were selected as study subjects.The subject 1 was a 27-year-old female,and subject 2 was a 40-year-old male.The ABO blood group serological identification of subjects was performed by test tube method.PCR-sequence specific primer(SSP)method was used for ABO genotyping of subjects.Exons 6 and 7 of subjects′ABO gene were sequenced by Sanger sequencing method.Procedure followed in this study was in line with requirements of the World Medical Association Declaration of Helsinki revised in 2013.Results①The serological phenotype of subject 1 was consistent with AwB or B(A)subtype,and subject 2 was consistent with A2B or B(A)subtype.②The ABO gene of subject 1 was heterozygous for B(A)02 and O,with a key nucleotide mutation c.700C>G in exon 7,and her ABO genotype was B(A).02/O.01.01.The ABO gene of subject 2 was heterozygous for B(A)04 and O,with a key nucleotide heterozygous mutation c.640A>G in exon 7,and his ABO genotype was B(A).04/O.01.01.Conclusions It is difficult to identify B(A)subtype using only blood group serological tests,and gene sequencing can provide a molecular basis for serological test results.

关 键 词：ABO血型系统 血清学试验 序列分析 DNA 血型鉴定和交叉配血 B(A)亚型 分子生物学 

分 类 号：R457.11[医药卫生—治疗学]