Hydrogenase as the basis for green hydrogen production and utilization  

作　　者：Haishuo Ji Lei Wan Yanxin Gao Ping Du Wenjin Li Hang Luo Jiarui Ning Yingying Zhao Huangwei Wang Lixin Zhang Liyun Zhang 

机构地区：[1]State Key Laboratory of Medicinal Chemical Biology,College of Pharmacy,Nankai University,Tianjin 300350,China [2]Max Planck Institute for Chemical Energy Conversion,Stiftstrasse 34-36,45470 Mülheim an der Ruhr,Germany [3]State Key Laboratory of Medicinal Chemical Biology,College of Life Sciences,Nankai University,Tianjin 300350,China [4]State Key Laboratory of Bioreactor Engineering,and School of Biotechnology,East China University of Science and Technology(ECUST),Shanghai 200237,China

出　　处：《Journal of Energy Chemistry》2023年第10期348-362,I0011,共16页能源化学（英文版）

基　　金：supported by the National Key Research and Development Program of China (Nos. 2020YFA0907300, 2020YFA0907800);the National Natural Science Foundation of China (No. 22077069);the Natural Science Foundation of Tianjin (Nos. 19JCZDJC33400 and 21JCYBJC00310);the Fundamental Research Funds for the Central Universities, Nankai University (No. 63201111)。

摘　　要：Hydrogenase is a paradigm of highly efficient biocatalyst for H_(2) production and utilization evolved in nature. A dilemma is that despite the high activity and efficiency expected for hydrogenases as promising catalysts for the hydrogen economy, the poor oxygen tolerance and low yield of hydrogenases largely hinder their practical application. In these years, the enigmas surrounding hydrogenases regarding their structures, oxygen tolerance, mechanisms for catalysis, redox intermediates, and proton-coupled electron transfer schemes have been gradually elucidated;the schemes, which can well couple hydrogenases with other highly efficient(in)organic and biological catalysts to build novel reactors and drive valuable reactions, make it possible for hydrogenases to find their niches. To see how scientists put efforts to tackle this issue and design novel reactors in the fields where hydrogenases play crucial roles, in this review,recent advances were summarized, including different strategies for protecting enzyme molecules from oxygen, enzyme-based assembling systems for H_(2) evolution in the photoelectronic catalysis, enzymatic biofuel cells for H_(2) utilization and storage and the efficient electricity-hydrogen-carbohydrate cycle for high-purity hydrogen and biofuel automobiles. Limitations and future perspectives of hydrogenasebased applications in H_(2) production and utilization with great impact are discussed. In addition, this review also provides a new perspective on the use of biohydrogen in healthcare beyond energy.

关 键 词：HYDROGENASE Biological H_(2)energy Oxygen tolerance Artificial photosynthesis Biofuel cells 

分 类 号：TQ116.2[化学工程—无机化工] TQ426.97