基于胸腺基质淋巴细胞生成素/蛋白酶激活受体2/瞬时电位受体香草酸受体1/活化T细胞的核因子信号通路探讨麻荆止咳颗粒治疗咳嗽变异性哮喘的作用机制  

A study on the mechanism of the Majing Zhike granules in treating cough variant asthma based on TSLP/PAR2/TRPV1/NFAT signal pathway

作　　者：陈家卫[1] 蔡穗珍[1] CHEN Jiawei

机构地区：[1]福建中医药大学附属第二人民医院,福建福州350003

出　　处：《中医临床研究》2023年第28期69-74,共6页Clinical Journal Of Chinese Medicine

基　　金：2020年度福建省自然科学基金资助项目(2020J01254),项目名称:基于TSLP/PAR2/TRPV1/NFAT信号通路探讨麻荆止咳颗粒治疗咳嗽变异性哮喘的作用机制,项目负责人:陈家卫。

摘　　要：目的:通过麻荆止咳颗粒调控胸腺基质淋巴细胞生成素(Thymic Stromal Lymphopoietin,TSLP)/蛋白酶激活受体2(Proteinase Activated Receptor-2,PAR2)/瞬时电位感受器香草酸受体1(Transient Receptor Potential Vanilloid 1,TRPV1)/活化T细胞核因子(Nuclear Factor of Activated T cells,NFAT)信号通路的动物实验,探讨其治疗咳嗽变异性哮喘(Cough Variant Asthma,CVA)的疗效机制。方法:将豚鼠随机分为空白对照组、模型组、中药组和地塞米松组,构建CVA豚鼠模型,药物干预10天,采用辣椒素激发各组豚鼠,观察咳嗽情况;苏木精-伊红(Hematoxylin-Eosin,HE)染色法观察各组肺组织病理学变化;酶联免疫吸附试验(Enzyme-Linked Immunosorbent Method,ELISA)法检测各组血清TSLP含量;免疫组化染色及实时定量聚合酶链反应(quantitative Real-time Polymerase Chain Reaction,qRT-PCR)法检测肺组织PAR2、TRPV1、NFAT的表达。结果:与模型组对比,中药组和地塞米松组药物干预后咳嗽次数明显减少;血清TSLP含量显著降低;且肺组织病理变化较模型组明显减轻;PAR2、TRPV1和NFAT的蛋白与mRNA表达均下调。结论:麻荆止咳颗粒可通过抑制CVA模型豚鼠TSLP/PAR2/TRPV1/NFAT信号通路表达,降低气道敏感性及改善气道炎症,达到止咳效果。Objective:To investigate the efficacy mechanism of the Majing Zhike granules(麻荆止咳颗粒)on cough variant asthma(CVA)by modulating thymic stromal lymphopoietin(TSLP)/proteinase activated receptor-2(PAR2)/transient receptor potential vanilloid 1(TRPV1)/nuclear factor of activated T cells(NFAT)signaling pathway through the animal experiment.Methods:Guinea pigs were randomly divided into the blank control group,the model group,the TCM medicine group andO the dexamethasone group.The guinea pig model of CVA was established.After 10 days of drug intervention,the guinea pigs in each group were stimulated with capsaicin to observe the cough.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of lung tissue in each group.Serum TSLP was detected by enzyme-linked immunosorbent method(ELISA),immunohistochemical staining and quantitative real-time polymerase chain reaction(qRT-PCR)were used to detect the expression of PAR2,TRPV1 and NFAT.Results:Compared with the model group,the number of coughs was significantly reduced in the TCM medicine group and the dexamethasone group after drug intervention,serum TSLP levels were significantly lower,and lung histopathological changes were significantly reduced,and protein and mRNA expressions of PAR2,TRPV1,NFAT were down-regulated.Conclusion:The Majing Zhike granules can inhibit TSLP/PAR2/TRPV1/NFAT signal pathway in CVA guinea pig model,reduce the sensitivity and inflammation of airway sensitivity,and achieve the effect on relieving cough.

关 键 词：麻荆止咳颗粒 咳嗽变异性哮喘 胸腺基质淋巴细胞生成素/蛋白酶激活受体2/瞬时电位受体香草酸受体1/活化T细胞的核因子信号通路 气道敏感性 

分 类 号：R256.12[医药卫生—中医内科学]