喉咽清口服液联合重组人干扰素α-2b口腔喷雾剂治疗手足口病的疗效及对炎性因子、免疫功能的影响  

Effect of Houyanqing oral liquid combined with recombinant human interferonα-2b oral spray on hand,foot and mouth disease and its effect on inflammatory factors and immune function

作　　者：冉振兵 Ran Zhenbing(Department of Pediatrics,Wanzhou Maternal and Child Health Hospital,Chongqing 404000,China)

机构地区：[1]重庆市万州区妇幼保健院,重庆404000

出　　处：《首都食品与医药》2023年第21期60-63,共4页Capital Food Medicine

摘　　要：目的探讨喉咽清口服液联合重组人干扰素α-2b口腔喷雾剂治疗手足口病的疗效及对炎性因子、免疫功能的影响.方法将2020年1月-2022年6月收治的80例手足口病患儿按随机数字表法分为对照组和观察组,每组40例,对照组患儿使用重组人干扰素α-2b口腔喷雾剂对咽后壁、双侧扁桃体及口腔内病灶处进行喷雾治疗,观察组在对照组的基础上再口服喉咽清口服液,两组患儿均治疗5d,记录两组患儿临床体征消失时间(退热、皮疹消退、口腔溃疡),治疗前及治疗5d结束时采用ELISA法测定炎症因子水平(C-反应蛋白、白细胞介素-4),采用流式细胞仪测定CD4^(+)、CD8^(+),计算CD4^(+)/CD8^(+)比值,采用多克隆抗体单向免疫扩散法测定免疫球蛋白水平,治疗5d结束时进行临床疗效评价.结果观察组退热时间、皮疹消退时间、咽部疱疹消退时间、口腔黏膜溃疡愈合时间分别为(1.20±0.45)d、(3.58±0.62)d、(2.04±0.36)d、(2.16±0.45)d,明显短于对照组[(1.58±0.40)d、(5.33±1.06)d、(4.25±0.54)d、(4.46±0.66)d],均P<0.05.观察组治疗后C-反应蛋白、白细胞介素-4分别为(2.02±0.38)mg/L、(14.56±2.82)ng/L,明显低于治疗前[(6.12±1.18)mg/L、(28.33±4.16)ng/L]及对照组治疗后[(3.85±0.64)mg/L、(20.32±3.70)ng/L],均P<0.05.观察组治疗后CD4^(+)、CD4^(+)/CD8^(+)分别为(47.83±7.05)%、(1.52±0.28),明显高于治疗前[(38.46±5.52)%、(0.98±0.16)]及对照组治疗后[(43.12±6.08)%、(1.20±0.24)],P<0.05,观察组治疗后CD8^(+)为(31.70±4.02)%,明显低于治疗前(39.26±6.54)%及对照组治疗后(35.85±4.60)%,P<0.05.观察组治疗后IgG、IgM、IgA分别为(12.78±2.13)g/L、(1.50±0.24)g/L、(1.38±0.20)g/L,明显高于治疗前[(6.20±1.06)g/L、(0.88±0.15)g/L、(0.55±0.10)g/L]及对照组治疗后[(8.88±1.32)g/L、(1.14±0.20)g/L、(0.96±0.18)g/L],均P<0.05.观察组临床总有效率为95%(38/40),明显高于对照组的80%(32/40),χ^(2)=6.441,P<0.05.结论在重组人干扰素αObjective To investigate the curative effect of Houyanqing oral liquid combined with recombinant human interferonα-2b oral spray in the treatment of hand,foot and mouth disease(HFMD)and its effects on inflammatory factors and immune function.Methods A total of 80 children with hand,foot and mouth disease treated from January 2020 to June 2022 were divided into control group and observation group according to a random number table,with 40 cases in each group.In the control group,the retropharyngeal wall,bilateral tonsils and oral lesions were treated with recombinant human interferonα-2b oral spray.In the observation group,Houyanqing oral liquid was taken orally on the basis of the control group.The children in both groups were treated for 5 days,and the disappearance time of clinical signs(fever,rash,and oral ulcer)was recorded in both groups.The levels of inflammatory factors(C-reactive protein,interleukin-4)were measured by ELISA before treatment and at the end of 5 days.CD4^(+)and CD8^(+)were measured by flow cytometry,and the ratio of CD4^(+)/CD8^(+)was calculated.The immunoglobulin level was determined by polyclonal antibody unidirectional immunodiffusion method,and the clinical efficacy was evaluated at the end of 5 days of treatment.Results In the observation group,the time of fever regression,rash regression,pharyngeal herpes regression and oral mucosal ulcer healing were(1.20±0.45)d,(3.58±0.62)d,(2.04±0.36)d and(2.16±0.45)d,respectively.It was significantly shorter than the control group[(1.58±0.40)d,(5.33±1.06)d,(4.25±0.54)d,(4.46±0.66)d],all P<0.05.After treatment,the levels of C-reactive protein and interleukin-4 were(2.02±0.38)mg/L and(14.56±2.82)ng/L,respectively.Significantly lower than before treatment[(6.12±1.18)mg/L,(28.33±4.16)ng/L]and after treatment[(3.85±0.64)mg/L,(20.32±3.70)ng/L],all P<0.05.CD4^(+)and CD4^(+)/CD8^(+)after treatment were(47.83±7.05)%and(1.52±0.28),respectively,significantly higher than before treatment[(38.46±5.52)%,(0.98±0.16)]and after treatment[(43.12

关 键 词：手足口病 喉咽清口服液 重组人干扰素Α-2B 临床研究 

分 类 号：R512.5[医药卫生—内科学]