霍乱监测中4号琼脂、TCBS琼脂及庆大霉素琼脂优劣势对比  

Comparison of Advantages and Disadvantages of No.4 Agar,TCBS Agar and Gentamicin Agar in Cholera Surveillance

作　　者：刘芷含 LIU Zhi-han(Laboratory Department of Fuliang County Center for Disease Control and Prevention,Fuliang 333400,Jiangxi,China)

机构地区：[1]浮梁县疾病预防控制中心检验科,江西浮梁333400

出　　处：《医学信息》2023年第22期148-151,共4页Journal of Medical Information

摘　　要：目的对比4号琼脂、TCBS琼脂及庆大霉素琼脂在霍乱监测中的优劣势。方法选取2022年1月-2023年3月浮梁县疾病预防控制中心检验科分离保存的霍乱弧菌株,增菌后分别接种于4号琼脂、TCBS琼脂及庆大霉素琼脂三种培养基中进行分离培养,比较3种培养基成分,观察霍乱弧菌在不同培养基中的菌落特征,统计并对比其检测结果。结果4号琼脂、TCBS琼脂与庆大霉素琼脂培养基配方成分存在一定差异,其中TCBS琼脂培养基成分种类最多,但三者琼脂含量相同,其pH值无差异(P>0.05)。霍乱弧菌在4号琼脂、TCBS琼脂与庆大霉素琼脂中的菌落生长特征存在一定差异。本次霍乱弧菌检出率由高至低依次为4号琼脂>庆大霉素琼脂>TCBS琼脂(P<0.05)。结论4号琼脂、TCBS琼脂与庆大霉素琼脂培养基均可有效分离霍乱弧菌,实现病菌检测,其营养基成分不同、菌落生长表现各异,以4号琼脂培养基的检出效果最佳。Objective To compare the advantages and disadvantages of No.4 agar,TCBS agar and gentamicin agar in cholera surveillance.Methods From January 2022 to March 2023,the strains of Vibrio cholerae isolated and preserved by the Laboratory Department of Fuliang County Center for Disease Control and Prevention were inoculated into No.4 agar,TCBS agar and gentamicin agar respectively after enrichment for isolation and culture.The composition of the three media was compared,the colony characteristics of Vibrio cholerae in different media were observed,and the detection results were statistically compared.Results There were some differences in the composition of No.4 agar,TCBS agar and gentamicin agar culture medium.Among them,TCBS agar culture medium had the most kinds of ingredients,but their agar contents were the same,and their pH values had no statistical difference(P>0.05).The colony growth characteristics of Vibrio cholerae in No.4 agar,TCBS agar and gentamicin agar were different.The detection rate of Vibrio cholerae in this study was ranked from high to low in the order of No.4 agar,gentamicin agar,and TCBS agar(P<0.05).Conclusion No.4 agar,TCBS agar and gentamicin agar culture medium can effectively isolate Vibrio cholerae and realize the detection of the bacteria.The composition of the nutrient medium is different,and the colony growth performance is different.The detection effect of No.4 agar culture medium is the best.

关 键 词：霍乱弧菌 4号琼脂 TCBS琼脂 庆大霉素琼脂 培养基成分 病菌检出率 

分 类 号：R378.3[医药卫生—病原生物学]