2018-2021年长沙地区自然人群隐匿性乙型肝炎病毒感染状况及S区变异分析  

Infection situation and S gene mutation of occult hepatitis B virus infection among natu population in Changsha from 2018 to 2021

作　　者：王远懿 张毕明 候正利 杨茜 阙正书 WANG Yuanyi;ZHANG Biming;HOU Zhengli;YANG Qian;QUE Zhengshu(Laboratory Department of Changsha Fourth Hospital,Changsha 410006,China)

机构地区：[1]长沙市四医院检验科,长沙410006

出　　处：《湖南师范大学学报（医学版）》2023年第4期60-64,共5页Journal of Hunan Normal University(Medical Sciences)

基　　金：湖南省自然科学基金科卫联合项目(2019JJ80110)。

摘　　要：目的:了解长沙地区2018-2021年自然人群隐匿性乙型肝炎病毒(occult hepatitis B virus infection,OBI)感染状况,探讨HBV基因型分布特征及S区氨基酸变异特点.方法:收集2018年9月—2021年12月长沙地区血清样本共68,513份,分析OBI流行病学特征;将确诊OBI样本进行血清学和高敏病毒载量检测,采用巢式PCR对其S区基因扩增并测序,使用MEGA6.0软件进行HBV基因分型及S区氨基酸突变分析.结果:68,513份样本中共检出27例OBI样本,OBI感染率为0.039%;长沙地区不同年龄人群的OBI感染率不同,其中41~79岁年龄组OBI感染率最高;发现5种血清学模式,抗HBc阳性率为88.90%;HBV-DNA核酸定量范围(9~191)IU/mL,(中位数值19 IU/mL);8例样本扩增出S区序列,基因型均为B型,均在HBsAg主要亲水区(major hydrophilic region,MHR)发生氨基酸突变,且集中在"a"抗原决定簇区域.结论:2018-2021年长沙地区自然人群中OBI感染率偏低,HBV基因型主要是B型.HBV S区MHR的氨基酸变异,尤其是"a"决定簇突变可能是造成OBI发生的重要因素之一.Objective To research the prevalence of the occult hepatitis B virus infection(OBI)among the natural population in Changsha from 2018 to 2021 and to explore the genotype distribution of virus strain and the mutation of amino acid sequence in S region,so as to provide an epidemiological and molecular biological basis for exploring the prevention and control of OBI in this region.Methods A total of 68,513 serum samples from september 2018 to december 2021 were collected,Among them,OBI samples were screened,yield and their epidemiological characteristics were analyzed.Confirmed OBI samples will be tested for serology and high-susceptibility viral load testing,and were genetically amplified in the S region by nested PCR amplification and sequenced,HBV gentyping and amino acid mutation analysis in the S-region were performed using MEGA6.0 software.Results 27 OBI samples were confirmed,the detection rate of OBI was 0.039%;5 OBI serological patterns were found,anti-HBc positive rate was 88.90%in all samples;HBV DNA nucleic acid quantitative ranged from 9.0 to 191.0(IU/mL)(median value of 19.0);8 samples of those OBI donors were amplified for S region,allthose are B genotype,which was shown amino acid mutation in MHR region of S gene,especially in HBV"a"epitope.Conclusion The OBI rate of natural population in Changsha is at low level in china(0.0106%-2.71%)from 2018 to 2021;The genotype of HBV is mainly type B,The mutation in MHR region of S gene,especially in HBV"a"epitope may be one of the important factors to cause OBI.

关 键 词：隐匿性乙型肝炎病毒 自然人群 乙肝病毒基因型 变异分析 

分 类 号：R512.6[医药卫生—内科学] R183[医药卫生—临床医学]