Human bocavirus 1 and 2 genotype-specific antibodies for rapid antigen testing in pediatric patients with acute respiratory infections  

作　　者：Ri De Yan-Peng Xu Fang Wang Yu-Tong Zhou Pan-Deng Shi Ru-Nan Zhu Yu Sun Li-Ying Liu Li-Ping Jia Hui-Jin Dong Hui Zhao Cheng-Feng Qin Lin-Qing Zhao 

机构地区：[1]Laboratory of Virology,Beijing Key Laboratory of Etiology of Viral Diseases in Children,Capital Institute of Pediatrics,Beijing 100020,China [2]State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing,100071,China

出　　处：《World Journal of Pediatrics》2023年第10期1009-1016,共8页世界儿科杂志（英文版）

基　　金：supported by the Beijing Natural Science Foundation(7192029);the National Natural Science Foundation of China(82172277);the Beijing Municipal Commission of Health(2060399 PXM2017_026268_00005_00254486).

摘　　要：Background Previous serological studies of human bocavirus(HBoV)1 could not exclude cross-reactivity with the other three HBoVs,particularly HBoV2.Methods To search for genotype-specific antibodies against HBoV1 and HBoV2,the divergent regions(DRs)located on the major capsid protein VP3 were defined through viral amino acid alignment and structure prediction.DR-deduced peptides were used as antigens to harvest corresponding anti-DR rabbit sera.To determine their genotype specificities for HBoV1 and HBoV2,these sera samples were used as antibodies against the antigens VP3 of HBoV1 and HBoV2(expressed in Escherichia coli)in western blotting(WB),enzyme-linked immunosorbent assay(ELISA),and bio-layer interferometry(BLI)assays.Subsequently,the antibodies were evaluated with clinical specimens from pediatric patients with acute respiratory tract infection by indirect immunofluorescence assay(IFA).Results There were four DRs(DR1–4)located on VP3 with different secondary and tertiary structures between HBoV1 and HBoV2.Regarding the reactivity with VP3 of HBoV1 or HBoV2 in WB and ELISA,high intra-genotype cross-reactivity of anti-HBoV1 or HBoV2 DR1,DR3,and DR4,but not anti-DR2,was observed.Genotype-specific binding capacity of anti-DR2 sera was confirmed by BLI and IFA,in which only anti-HBoV1 DR2 antibody reacted with HBoV1-positive respiratory specimens.Conclusion Antibodies against DR2,located on VP3 of HBoV1 or HBoV2,were genotype specific for HBoV1 and HBoV2,respectively.

关 键 词：Divergent regions Genotype-specific antibody Human bocavirus 1 and 2 Major capsid protein VP3 

分 类 号：S85[农业科学—兽医学]