微生物诱导碳酸盐沉淀修复铅(Pb)污染黄土的试验研究  

Experimental study on remediation of lead(Pb) contaminated loess usingmicrobial⁃induced carbonate precipitation (MICP)

作　　者：张斌[1,2] 郑文杰[1,2] 薛中飞 王琳 秦鹏[1,2] 何文杰[1,2] Zhang Bin;Cheng Wen-chieh;Xue Zhongfei;Wang Lin;Qin Peng;He Wenjie(School of Civil Engineering,Xi’an University of Architecture and Technology,Xi’an 710055,China;Shaanxi Key Laboratory of Geotechnical and Underground Space Engineering(XAUAT),Xi’an 710055,China)

机构地区：[1]西安建筑科技大学土木工程学院,陕西西安710055 [2]西安建筑科技大学陕西省岩土与地下空间工程重点实验室,陕西西安710055

出　　处：《土木工程学报》2023年第11期114-123,共10页China Civil Engineering Journal

基　　金：创新人才推进计划-科技创新团队(2020TD-005)。

摘　　要：重金属污染是一个世界性的环境问题,对人类健康和野生动物都有潜在的严重影响。文章首先采用不同菌液接种比例(1∶9,1∶3)进行了溶液中的铅(Pb)修复试验。另外利用Visual MINTEQ软件研究了碳酸盐沉淀的形态和其生成次序。最后进行黄土中Pb修复试验。结果表明,细菌接种比例为1∶3时Pb修复效率达到96%。在后续开展的土柱修复试验中,试验结果发现注入菌液量测得出的细菌活性随菌液渗透距离的增加而降低,而且黄土本身就可以与一部分Pb结合,将溶液中的游离态Pb转换为碳酸盐态。在Pb污染质量分数为1250mg/kg的黄土中,巴氏芽孢八叠球菌修复后碳酸盐态的Pb质量分数提升9.71%。在Pb污染质量分数500mg/kg时其土柱上部修复效率达到了97.41%,土柱下部修复效率达80.71%。这些发现突显了MICP技术应用于Pb污染黄土修复的潜力。Heavy metal pollution is a worldwide environmental problem,which has a potential serious impact on human health and wildlife.In this study,different bacterial inoculation ratios(1∶9,1∶3)were used to carry out the Pb remediation test.In addition,visual MINTEQ software was used to study the morphology and formation sequence of carbonate precipitation.Finally,the Pb remediation test in loess was carried out.The results showed that the Pb remediation efficiency reached 96%when the ratio of bacteria inoculation was 1∶3.In the subsequent soil column restoration test,the bacterial activity measured by the injected bacterial solution decreased with the penetration distance of the bacterial solution,and it was observed that the Loess could combine with a part of Pb to convert the free Pb in the solution into carbonate.In the Loess with a Pb pollution concentration of 1250mg/kg,the content of Pb in carbonate state increased by 9.71%after the restoration of MICP.When the Pb pollution concentration was 500mg/kg,the remediation efficiency of the upper part of the soil column reached 97.41%,and the remediation efficiency of the lower part of the soil column reached 80.71%.The MICP technology showed great potential in the remediation of Pb contaminated loess.

关 键 词：微生物矿化 铅(Pb)污染 巴氏芽孢八叠球菌 脲酶活性 修复效率 

分 类 号：TU411[建筑科学—岩土工程]